成都大亭 CHENGDU UNIVERSITY 份尊们 Select gene cluster 4oee3060090u00090000@21@224262 Identified secondary metabolite dusters Cluster From To Most similar known duster MIBIG BGC-ID The following clusters are from record NC_0038883 Cluster 1 Tlpks-Otherks 86637 139654 Leinamycin_ biosynthetic_gene _cluster(2% of genes show similarity) BGC0001101c1 Cluster 2Terpene BGC0000664c1 235986 271084 Sanglifehrin_a- biosynthetic_gene_ cluster(4% of genes show similarity) Cluster 4 493989 544920 Coelichelin_biosynthetic _gene_cluster(100% of genes show similarity) BGC0000325c1 Bacteriocin 791 ormatipeptin- biosynthetic_ _gene_cluster(28% of genes show similarity BGC0000518c1 Cluster 6 1257625 1298749 Herboxidiene_biosynthetic_genecluster(8% of genes show similarity Cluster 7 Ectoine 1995500 2005898 Ectoine biosynthetic_gene _cluster(100% of genes show similarity) BGC0000853c1 Cluster 8 Melanin 2939306 2949875 Lactonamycin_ biosynthetic_gene_cluster (3% of genes show similarity) BGC0000238c1 Cluster 9 Siderophore 3895 3045682 Desferrioxamine_b_ biosynthetic_gene_cluster(83% of genes show similarity) Cluster 10Nrps 3523335 3603988 Calcium-dependent_antibiotic_biosynthetic_gene_ cluster(90%of genes show similarity) BGC0000315-_c1 Cluster 11 T2pks 5509801 5552424 Actinorhodin_ biosynthetic _gene_cluster(100% of genes show similarity) BGC0000194c1 Cluster12Terpen 5671016 5692101 Albaflavenone_ biosynthetic_gene_cluster(100% of genes show similarity Cluster 13 T2pks 5766945 5809487 Spore_pigment biosynthetic_gene _cluster(66% of genes show similarity) BGC0000271c1 Cluster 14 Siderophore 63355876347533 6429549 6476442 Undecylprodigiosin_ biosynthetic_gene_cluster(95% of genes show similarity BGC0001063c1 Cluster 16 Bacteriocin 66323436643659 Cluster 17 Terpene 66562196678399 Cluster 18Siderophore 6842315 6855522 Enduracidin_biosynthetic_gene_ cluster(6% of genes show similarity BGC000341c1 Cluster 19 Tlpks-Butyrolactone 6871293 6951959 Coelimycin_ biosynthetic_gene_cluster(95% of genes show similarity BGC0000038c1 Cluster 20Nrps 7088264 7136089 Nogalamycin_biosynthetic_ gene_cluster(40% of genes show similarity) BGC000249c1 Cluster 21 Antipeptide 7409664 7432456 SAL-2242_biosynthetic_gene_cluster(80% of genes show similarity) BGC0000546c1 Cluster 22Terpene 7506017 7532758 Hopene_biosynthetic_genecluster(92% of genes show similarity) BGC0000663c1 Cluster 23 Tlpks-Otherks 7570412 7618555 Arsenopolyketides_ biosynthetic _gene_cluster(95% of genes show similarity BGC0001283c1 uster 24 Antipeptide 76850167709795 Cluster 25 Othe 79734708014357 Cluster 26 Indole 8269637 8290764 Ravidomycin_ biosynthetic_gene_cluster(5% of genes show similarity BGC0000263c1 Cluster 27 T3pks-Terpene-Nrps 847 75102 8558352 Coelibactin_ biosynthetic_ gene_cluster(100% of genes show similarity) BGC0000324_c1
成都大亭 CHENGDU UNIVERSITY 为1份自尊 NC 003888- Cluster 4- Nrps Gene cluster description NC_003888-Gene Cluster 4. Type =nrps. Location: 493989-544920 nt Cluster Finder probability: 0.9002. Click on genes for more information Show pHMM detection rules used KKDXKDWXXI ■核心的合成基因 Query sequence D①Da 口额外的合成基因 AL939105-c1: Streptomyces coelicolor A3(2) complete genome; segment 2/29.(100% of genes show similarity) CKKKDIKDDEXO KD□ 运输相关的基因 CP009124_c22: Streptomyces lividans TK24, complete genome.(100% of genes show similarity) CKK KDHXK 争D 调节基因 JXWX01000001-c1: Streptomyces sp, WM6391 contigl I, whole genome shotgun se,.(97% of genes show similanity KKDXXK 一DD D 其他基因 CM001889_c4: Streptomyces lividans 1326 chromosome, whole genome shotgun se.(100% of genes show similarity ③的D PD匚 GG657756-_c21: Streptomyces lividans TK24 genomic scaffold supercont1. 1, who,,(102% of genes show similan 心③DD DD 和同源基因簇比对的结 LGKF01001112-_c1: Streptomyces sp NRRL WC-3753 WC3753contig2. 1, whole genom,,(68% of genes show similanity) D和Dp KKKKKID+ DDL 果示意图,还有比对的 LGCX01000236_c1: Streptomyces sp NRRL F-4711 P348contig51, whole genome s.(57% of genes show similarity) 一 DD-LDDDD 相似度。 LGEH01000149_c1: Streptomyces sp NRRL F-7442 P447contig32. 1, whole genome ...(57% of genes show similarity) DD-CLD-DD)DDDC KKKKKIK DLDDDD- LGEF01000173-_c1: Streptomyces sp NRRL F-4707 p445contig7, 1, whole genome s,.(57% of genes show similanity) DDD-IDDDDO >EDD AHGS01000163_c1: Streptomyces coelicoflavus ZG0656 contigo0106, whole geno.(47% of genes show similarity) -KK CKKKKKD+ DDLDDDD CHENGDU UNⅣ ERSITY
核心的合成基因 额外的合成基因 运输相关的基因 调节基因 其他基因 和同源基因簇比对的结 果示意图，还有比对的 相似度
成都大亭 CHENGDU UNIVERSITY 份尊们 Homologous known gene dusters All hits Predicted core structure Query sequence Chiral NH2 BGC0000325: Coelichelin biosynthetic gene cluster(100% of genes show similarity H2N K① BGC0000336: Daptomycin biosynthetic gene cluster (54% of genes show similarity) D KXKKXXDAKKX rediction of core scaffold based on assumed KKKK4DDD-DDODCCPKS/NRPS colinearity; tailoring reactions not taken into BGC0000423: Scabichelin biosynthetic gene cluster(54% of genes show similarity) Prediction details Monomers prediction BGCo001211: Albachelin biosynthetic gene cluster(45% of genes show similarity) SCO0492 Stachelhaus code: thorn hornlorn NRPSPredictor 3 SVM BGCo000366: Gobichelin biosynthetic gene cluster(36% of genes show similarity) PrediCAT: Q9RK14_ Al-orn horn SANDPUMA ensemble: orn 243<米 ID to nearest neighbour: 100.0 SNN score:1.09134343917 BGCo001351: Cahuitamycins biosynthetic gene cluster(27% of genes show similarity) Stachelhaus code: athrlbmtld-athrId hrldhab dhabul dhtInme-thrItl NRPSPredictor 3 SVM: thr pHMM: thr PrediCAT: Q9RK14 A2-athr SANDPUMA ensemble: athr thr D to nearest neighbour: 100.0 Stachelhaus code: hornInme-fhorn NRPSPredictor 3 SVM: N/A pHMM: horn PredICAT: NA-n/a ANDPUMA ensemble: N/A %ID to nearest neighbour: 55.6 Search NORINE for peptide in strict mode // relaxed CHENGDU UNⅣ ERSITY
成都大亭 CHENGDU UNIVERSITY 为1份自尊 基本流程 确定目的基因 傅慧垚 刘亚洲 基因敲除 基因表达 张君利 日的基因的导入、检测与筛选}钟艾玲 产物分析 郭森林 CHENGDU UNⅣ ERSITY
确定目的基因 基因敲除 基因表达 目的基因的导入、检测与筛选 产物分析 傅慧垚 刘亚洲 张君利 钟艾玲 郭森林 基本流程
成都大亭 CHENGDU UNIVERSITY 为1份自尊 基因表达 CHENGDU UNⅣ ERSITY
基因表达