基因女库 基因组DNA文库 (genomic DNA library) ●cDNA文库 (CDNA library)
基因文库 • 基因组DNA文库 (genomic DNA library) • cDNA文库 (cDNA library)
human DNA CLEAVE WITH RESTRICTION NUCLEASE @ millions of genomic DNA fragments DNA FRAGMENTS INSERTED INTO PLASMIDS INTRODUCTION OF PLASMIDS INTO BACTERIA @a8g0 genomic library
Cellular Bacteriophages genome Cleave with Extract DNA. restriction enzyme. Cleave Ligate Recombinant DNA Hybrid phages as29 aa98e2ss分 IImIm
Bacterium Infection Lysis Plaque Lawn of bacterium Phage clone in plaque Library of phage clones es20sadeceassge
聚合酶链反(PCR) Polymerase Primer 1 +DNA Primer Primer 2 dNTPS Chain DNA-Polymerase Proben-DNA Denaturieren 95C Reaction Synthese~45C Cycle 1 。 基本原理： Denaturieren 95C Cycle 2 Synthese~45C 变性、 退火、 Cycle 3 延伸 10i
聚合酶链反应（PCR） • Polymerase Chain Reaction • 基本原理： 变性、 退火、 延伸