实验原理是染色体或染色单体的无着丝点断片或纺锤丝受损伤而丢失的整个染色体。>可检出DNA断裂剂和非整倍体诱变剂6
6 是染色体或染色单体的无着丝点断片或纺锤丝受 损伤而丢失的整个染色体。 可检出DNA断裂剂和非整倍体诱变剂。 实验原理
微核细胞形成过程骨髓外周血染色体断片药物处理网织红细胞正常红细胞I分裂成熟0正染红细胞分裂中期母红细胞分裂末期微核红细胞微核网织红细胞10h10h10h骨髓中检测到微核在18-30h（给药2次，通常在18-24h采样外周血液中检测到微核要>30h
微核细胞形成过程 10h 10h 10h 母红细胞 分裂中期 分裂末期 分裂 正常红细胞 微核红细胞 药物处理 染色体断片 网织红细胞 微核网织红细胞 成熟 正染红细胞 骨髓 外周血 骨髓中检测到微核在18-30h（给药2次，通常在18-24h采样） 外周血液中检测到微核要>30h
NucleusMicronucleusRats/micedosedwithcompound,3Micronucleimaybeformedbylossofwholechromosomedoses,6animals/group.Animalsduringdivisionorbychromosomebreakage.Theerythrocyte'ssacrificed24or48hourslaternucleusisextruded leavinganymicronucleibehindMicronucleusBonemarrowcellsspreadontoFemursremovedslides.Slidesfixedand stainedand bone marrow2000cellsanalysedperanimal,numberaspirated(acridine orange)ofmicronucleatedimmatureerythrocytesscored
Rats/mice dosed with compound, 3 doses, 6 animals / group. Animals sacrificed 24 or 48 hours later Micronuclei may be formed by loss of whole chromosome during division or by chromosome breakage. The erythrocyte’s nucleus is extruded leaving any micronuclei behind Nucleus Micronucleus Femurs removed and bone marrow aspirated Bone marrow cells spread onto slides. Slides fixed and stained (acridine orange) 2000 cells analysed per animal, number of micronucleated immature erythrocytes scored Micronucleus
Rodentbone-marrowmicronucleustestNCE
Rodent bone-marrow micronucleus test
5012吖啶橙染色后显示为肥大颗粒
吖啶橙染色后显示为肥大颗粒