复习-DNA甲基化2016
复习-DNA甲基化 2016
S复习-焦磷酸测序的技术原理在亚硫酸盐的作用下，所有未甲基化的胞嘧啶发生脱氨基反应转变成了尿嘧啶，但是5-甲基胞嘧啶不发生转变l.Bisulfitetreatmentof denaturedDNAmcmcCU2.PCRamplificationmCCUT一
3PolymeraseAGGATCTGAGCASTINY5TCCTAGACACCnaPPiCSulfurylaseApyraseATPcCLuciferaseLightQiagenQ96IDTime
A甲基化定焦磷酸测量序聚合酶链式反应亚硫酸(PCR)盐转化基因组DNA提取
Epilect BisulfiteConversionProcedure亚硫酸盐转化DNAond EpilectBisulite reoctionreaction buffersDNAconversion inthermal cycler (5 h)Transfer to 1.5ml microtubeCAdd BufferBL+ffffLood Spin ColumnBindWashAdd Buffer BW纯化DesulfonafionAdd BufferBDWash (× 2)AddBufferBW口Elute