。o Sequence of morphological changes in apoptosis. A morphologic
(B) DNA fragmentation Chromatin condensation Fragmentation of nucleus Fragmentation of the cell
(b) Nucleus Mild convolution Chromatin compaction and Condensation of ○ Nuclear fragmentation e Blebbing Cell fragmentation Phagocytosis Apoptotic body Phagocytic cell
Mild convolution Nuclear Chromatin fragmentation compaction and Blebbing Apoptotic Phagocytic segregation body cell Cell fragmentation Condensation of cytoplasm Q Phagocytose FIGURE 23-45(a): Ultrastructural features of cell death by apoptosis. (a)Schematic drawings Illustrating the progression of morphologic changes observed In apoptotic cells. Early in apoptosis, dense chromosome condensation occurs along the nuclear periphery. The cell body also shrinks although most organelles remain intact Later both the nucleus and cytoplasm fragment, forming apoptotic bodies. These are phagocytosed by surrounding cells [Part (a)adapted from J Kuby, 1997, mmunology, 3d ed, W H. Freeman Co, p 53.1
1234567 细胞色素c诱导的凋亡细胞DNA电泳图 细胞色素c诱导0h -2kb 2.细胞色素c诱导1h 3.细胞色素c诱导2h 1kb 4.细胞色素c诱导3h 5.细胞色素c诱导4h 500bp6.阴性对照 7. Marker 200bp (自赵允、翟中和)
细胞色素c诱导的凋亡细胞DNA电泳图 1．细胞色素c诱导0 h 2．细胞色素c诱导1 h 3．细胞色素c诱导2 h 4．细胞色素c诱导3 h 5．细胞色素c诱导4 h 6．阴性对照 7．Marker （ 自赵允、翟中和）