Oxidative stress-MtDNA(sesea/s(eegie"p<0.05 control vs AD"p<0.05 control vs.AD0ES2001002CERBLMFRCNTALPARIETALTEMPCRALCERBLMFRCNTALPARIETALTEMPORALnucloarDNAof controlnuclearCNAofconitrolnuclearDNA of ADnucloarCNA dl ADntDNAofconitrolmtDNAofconirolmIDNAofAD1mIDNAofADFig.3Meanregional differencesinlevelsof 8-hydroxyadenineFig.5Mean regional differences in levels of 5-hydroxycytosine.Significantelevationswere observedinnuclear DNA(nDNA)of5-Hydroxycytosine wassignificantlyelevated inAlzheimer'sdiseasefrontal (p<0.03)andparietal (p<0.04)lobes,andnDNA(p<0.01)(AD)samplesinnuclearDNA(nDNA)(p<0.01)andmitochondrialandmitochondrialDNA(mtDNA)(p<0.04)oftemporallobeDNA(mtDNA)(p<0.01)offrontallobe,mtDNA(p<0.01)ofparietalResultsareexpressedasmean±SEMalteredbases/1o6bases.lobeandnDNA(p<0.001)andmtDNA(p<0.05)oftemporallobe.*p<0.05.Results are expressed as mean±SEM altered bases/1o°bases.*p<0.05.JournalofNeurochemistry.2005,93,953-962
Oxidative stress-Mt DNA Journal of Neurochemistry, 2005, 93, 953–962
Oxidative stress-Mt DNAg*p<0.05controlvs.AD14p<0.05controlvs.AD120100SESUeusuepekdeoenoCERBLMFRONTALPARIETALTEMPORALCERBLMFRONTALPARIETALTEMPORALmuclaar DNA of controlnuclaarDNAofADmDNA of controlnucloar DNA of controlmDNAofADnuckoar DNAof ADmtDNA of controlmIDNAofADFig.4Meanregionaldifferencesinlevelsoffapyadenine.Significantelevationswere observedinnuclear DNA (nDNA)ofcerebellumFig.6Mean regional differences in the levels of 5-hydroxyuracil(p<0.02),mitochondrialDNA (mtDNA)ofparietal lobe(p<0.05),5-Hydroxyuracil was significantly elevated in mitochondrial DNAandnDNA(p<0.001)andmtDNA(p<0.05)oftemporallobe.(mtDNA)fromparietal(p<0.05)andtemporal(p<0.04)lobesofResults areexpressedasmean±SEMalteredbases/1obases.Alzheimer's disease (AD)subjects. Results are expressed as'p<0.05.mean±SEMalteredbases/10°bases.*p<0.05.JournalofNeurochemistry,2005,93,953-962
Oxidative stress-Mt DNA Journal of Neurochemistry, 2005, 93, 953–962
ArticleAPP-MtMitochondrial targeting and a novel transmembranearrest of Alzheimer's amyloid precursor proteinimpairs mitochondrial function in neuronal cellsImmunoelectronmicroscopeofHCN-1AcellstransfectedwithWT/APP
Immunoelectron microscope of HCN-1A cells transfected with WT/APP. APP-Mt
ArticleMitochondrial targeting and a novel transmembranearrest of Alzheimer's amyloid precursor proteinimpairs mitochondrial function in neuronal cellsVectorcontrol.yTransfeetedwith3M/APpAbeta inducedMTransfectedwith≥220-290/APPTransfectedwithWTiAPpTransfectedwithSWiAPpNeuronalA100mitochondrial(%damage50B6)432102496048HoursofTransfection
A beta induced Neuronal mitochondrial damage
ABADDirectlyLinksAβtoMitochondrialToxicityinAlzheimer'sDiseaseJovceW.Lustbader. etalScience304,448(2004):DOl:10.1126/science.10912301:1CAβABADmergeDVDACABADmerge(C)ColocalizationofABADandAincerebralcortexofADpatients(2OO-foldmagnification).(D)MitochondriallocalizationofABADincerebralcortexofADpatients(2o0-foldmagnification)ABAD:Amyloidβ.BindingAlcoholDehydrogenase
(C) Colocalization of ABAD and A in cerebral cortex of AD patients (200-fold magnification). (D) Mitochondrial localization of ABAD in cerebral cortex of AD patients (200-fold magnification). ABAD:Amyloid β. Binding Alcohol Dehydrogenase