CHAPTER 8 The replication of ona The mechanism of dNa Polymerase (Po|) Copyright@ 2004 Pearson Education, Inc, publishing as Benjamin Cummings 劣溪字 han universit
The mechanism of DNA Polymerase (Pol) CHAPTER 8 The replication of DNA
DNA Pol use a single active site to catalyze DNA synthesis a A single site to catalyze the addition e. of any of the four dNTPs. 3 Recognition of different dntp by 2 monitoring the ability of incoming 8 dNTP in forming A-T and G-C base pairs; incorrect base pair dramatically lowers the rate of catalysis(kinetic selectivity)
DNA Pol use a single active site to catalyze DNA synthesis A single site to catalyze the addition of any of the four dNTPs. Recognition of different dNTP by monitoring the ability of incoming dNTP in forming A-T and G-C base pairs; incorrect base pair dramatically lowers the rate of catalysis (kinetic selectivity). The mechanism of DNA Pol
a correct base pair b incorrect base pair template C-0—P-0 C-0-P-0 no base G pa dNTP dNTP OH OH OH prmer =P-0 =P-0 Copyright o 2004 Pearson Education, Inc, publishing as Benja Figure 8-3
Figure 8-3
Distinguish between rNTP and dNTP by steric exclusion of rNTPs from the active site 592392-9 template discrimeter amino scds a DNA polymerase Figure 8-4
Distinguish between rNTP and dNTP by steric exclusion of rNTPs from the active site. The mechanism of DNA Pol Figure 8-4
DNA Pol resemble a hand that grips the 3 primer-template junction Schematic of DNA pol bound to a primer;template junction 3 8 A similar view of the T7 DNA pol bound to dna Figure 8-5
DNA Pol resemble a hand that grips the primer-template junction The mechanism of DNA Pol Figure 8-5 Schematic of DNA pol bound to a primer:template junction A similar view of the T7 DNA pol bound to DNA