Molecular biology of the Gene 5/E - Watson et al. ( 2004) Part l: Chemistry and Genetics Part Maintenance of the Genome Part l: Expression of the Genome Part IV: Regulation Part va methods 刻博来字 Wuhan Univ
2 Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods
Part V: METHODS Ch 20: Techniques of Molecular Biology Ch 21: Model Organisms
3 Ch 20: Techniques of Molecular Biology Ch 21: Model Organisms Part V: METHODS
● Molecular iology course Chapter 20 Techniques of Molecular Biology Preparation, analysis and manipulation of nucleic acids and proteins
4 •Molecular Biology Course Chapter 20 Techniques of Molecular Biology Preparation, analysis and manipulation of nucleic acids and proteins
The methods depend upon, and were developed from, an understanding of the properties of biological macromolecules hemselves bridization---the base-pairing characteristics of dna and rna > DNA cloning--- DNA polymerase restriction endonucleases and dna ligase >PCR---Thermophilic DNA polymerase
5 The methods depend upon, and were developed from, an understanding of the properties of biological macromolecules themselves. ➢ Hybridization---the base-pairing characteristics of DNA and RNA ➢ DNA cloning--- DNA polymerase, restriction endonucleases and DNA ligase ➢ PCR---Thermophilic DNA polymerase
CATER20: Techniques of Molecular iolo Topⅰc1: Nucleic acids 1. Separation by Electrophoresis(电泳分离) 2 Cut by Restriction endonuclease(限制性 内切酶切割) 3工 dentification by Hybridization(杂交鉴 定) 4 PCR 5. Genome sequence analysis 6. DNA Cloning and gene expression
6 Topic 1: Nucleic acids CHAPTER20: Techniques of Molecular Biology 1.Separation by Electrophoresis (电泳分离) 2.Cut by Restriction endonuclease (限制性 内切酶切割) 3.Identification by Hybridization (杂交鉴 定) 4.PCR 5.Genome sequence & analysis 6.DNA Cloning and gene expression