fingers Thumb incoming nucleotide Fingers P template P 3 OH bend in template primer template base Palm Figure 8-8 Copyright C 2004 Pearson Education, Inc, publishiny ao uci allI l Cullllimllyo
Figure 8-8 Thumb Fingers Palm
DNA Polymerase-palm domain 1. Contains two catalytic sites, one for addition of dNTPs and one for removal of the mispaired dNTP 2. The polymerization site binds to two metal ions that alter the chemical environment around the catalytic site and lead to the catalysis (how?) 3. Monitors the accuracy of base-pairing for the most recently added nucleotides by forming extensive hydrogen bond contacts with minor groove of the newly synthesized dNA. ( See proofreading)
1. Contains two catalytic sites, one for addition of dNTPs and one for removal of the mispaired dNTP. 2. The polymerization site binds to two metal ions that alter the chemical environment around the catalytic site and lead to the catalysis. (how?) 3. Monitors the accuracy of base-pairing for the most recently added nucleotides by forming extensive hydrogen bond contacts with minor groove of the newly synthesized DNA. (See proofreading) DNA Polymerase-palm domain
DNA Polymerase-finger domain Binds to the incoming dNTP encloses the correct paired dNTP to the position for catalysis Bends the template to expose the only nucleotide at the template that ready for forming base pair with the incoming nucleotide Stabilization of the pyrophosphate
Binds to the incoming dNTP, encloses the correct paired dNTP to the position for catalysis Bends the template to expose the only nucleotide at the template that ready for forming base pair with the incoming nucleotide Stabilization of the pyrophosphate DNA Polymerase-finger domain
DNA Polymerase-thumb domain Not directly involved in catalysis Interacts with the synthesized dNA to maintain correct position of the primer and the active site, and to maintain a strong assoclation between dna Pol and its substrate
Not directly involved in catalysis Interacts with the synthesized DNA to maintain correct position of the primer and the active site, and to maintain a strong association between DNA Pol and its substrate. DNA Polymerase-thumb domain
DNA Pol are processive enzymes a Processivity is a characteristic of enzymes that operate on polymeric substrates The processivity of DNA Pol is the average number of nucleotides added z each time the enzyme binds a g primer: template junction(a iew-50,000)
DNA Pol are processive enzymes The mechanism of DNA Pol Processivity is a characteristic of enzymes that operate on polymeric substrates. The processivity of DNA Pol is the average number of nucleotides added each time the enzyme binds a primer:template junction (a few~50,000)