19.3 DNA is coiled in arrays of nucleosomes 200bp 165bp 146bp Mono Trimmed Core particles nucleosomes nucleosomes Figure 19.10 Microccocal nuclease initially cleaves between nucleosomes.Mononucleosomes typically have ~200 bp DNA. End-trimming reduces the length of DNA first to~165 bp,and then generates core particles with 146 bp 情華大当
Figure 19.10 Microccocal nuclease initially cleaves between nucleosomes. Mononucleosomes typically have ~200 bp DNA. End-trimming reduces the length of DNA first to ~165 bp, and then generates core particles with 146 bp. 19.3 DNA is coiled in arrays of nucleosomes
19.3 DNA is coiled in arrays of nucleosomes DNA"leaves Figure 19.4 The nucleosome may be a cylinder with DNA organized DNA "enters" into two turns around the surface. 清菜大兰
Figure 19.4 The nucleosome may be a cylinder with DNA organized into two turns around the surface. 19.3 DNA is coiled in arrays of nucleosomes
19.4 DNA structure varies on the nucleosomal surface Nick .NM八八八MNM八入NN入NN. 5'label Denature Nick 5'label Labeled fragm entm■量■■■量n Labeled tragment Figure 19.11 Nicks in double-stranded DNA are revealed by fragments when the DNA is denatured to give single strands.If the DNA is labeled at(say)5 ends,only the 5 fragments are visible by autoradiography.The size of the fragment identifies the distance of the nick from the labeled end. 清菜大当
Figure 19.11 Nicks in double-stranded DNA are revealed by fragments when the DNA is denatured to give single strands. If the DNA is labeled at (say) 5 ends, only the 5 fragments are visible by autoradiography. The size of the fragment identifies the distance of the nick from the labeled end. 19.4 DNA structure varies on the nucleosomal surface
19.4 DNA structure varies on the nucleosomal surface Figure 2.4 When Restriction map restriction fragments are identified by their Fragments identified on gel possession of a labeled A-G (unbroken) end,each fragment A-B-C-D-E-F directly shows the 会B8BE distance of a cutting site A-B-C from the end Successive fragments increase in length by the distance between adjacent restriction sites. Labeled end 清苇大当
Figure 2.4 When restriction fragments are identified by their possession of a labeled end, each fragment directly shows the distance of a cutting site from the end. Successive fragments increase in length by the distance between adjacent restriction sites. 19.4 DNA structure varies on the nucleosomal surface
19.4 DNA structure varies on the nucleosomal surface Figure 19.12 Sites for nicking lie at regular intervals along core DNA,as S4 seen in a DNAase I digest of nuclei. Photograph kindly provided by Leonard Lutter. 情華大当
Figure 19.12 Sites for nicking lie at regular intervals along core DNA, as seen in a DNAase I digest of nuclei. Photograph kindly provided by Leonard Lutter. 19.4 DNA structure varies on the nucleosomal surface