Laser tweezers are useful for lsolating slow-growing bacteria from mixed cultures Once the desired cell is far enough away from the Cell other cells,the capillary is Beam focus severed,the laser turned a b off,and the cell flushed Objective lens of microscope into a tube of sterile medium.一旦所需细胞远离其它 Laser beam 细胞，将毛细管切断，关闭激光，细胞 (a) 就可流出到培养基中 Optically trapped cell Severing point Capillary tube Mixture of cells (b) Laser Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University Laser tweezers are useful for Isolating slow-growing bacteria from mixed cultures Once the desired cell is far enough away from the other cells, the capillary is severed, the laser turned off, and the cell flushed into a tube of sterile medium.一旦所需细胞远离其它 细胞,将毛细管切断,关闭激光,细胞 就可流出到培养基中
ll.Culture-independent analyses of Microbial Communities Biodiversity reflected by DNA diversity DNA 样性反映了生物多样性 One cell-one chromosomal DNA 70%genomic homology and 97%homology in 16S rRNA as one species Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University II. Culture-independent analyses of Microbial Communities Biodiversity reflected by DNA diversity DNA的多 样性反映了生物多样性 • One cell-one chromosomal DNA • 70% genomic homology and 97% homology in 16S rRNA as one species