Mixture of △+△+△ and- charged ⊙④ Elute proteins Beads of + + charged + DEAE- cellulose 二乙氨基乙基纤维素 △+ 1→ Fraction number
Elute 二乙氨基乙基 纤维素
Ion-Exchange Chromatography 离子交换层析 4 0.5 0.4 3 0.3 2 0.2 0.1 10 20 30 Fraction number Figure 5.6 lon-exchange chromatography
Ion-Exchange Chromatography 离子交换层析
SUMMARY Ion-exchange chromatography can be used to separate substances,including proteins, according to their charges.Positively charged resins like DEAE-Sephadex are used for anion- exchange chromatography,and negatively charged resins like phosphocellulose are used for cation-exchange chromatography
SUMMARY Ion-exchange chromatography can be used to separate substances, including proteins, according to their charges. Positively charged resins like DEAE-Sephadex are used for anionexchange chromatography, and negatively charged resins like phosphocellulose are used for cation-exchange chromatography
Gel filtration chromatography(凝胶过滤层析)separates proteins on basis of their physical dimensions(size and shape)using porous(有孔的)beads packed in a column.This method can also be used to estimate the molecular mass of a protein. Large molecule Large molecules will not be able to enter any of the beads and will flow more quickly through the column. Small molecules will easily enter the holes in the Porous bead Small molecule beads and therefore flow through the column slowly
Large molecules will not be able to enter any of the beads and will flow more quickly through the column. Small molecules will easily enter the holes in the beads and therefore flow through the column slowly. Gel filtration chromatography（凝胶过滤层析）separates proteins on basis of their physical dimensions(size and shape) using porous(有孔的)beads packed in a column. This method can also be used to estimate the molecular mass of a protein
Small molecules Large molecules Gel filtration resins
Large molecules Small molecules Gel filtration resins