SUMMARY Gel filtration chromatography uses columns filled with porous resins that let in smaller substances,but exclude larger ones.Thus,the smaller substances are slowed in their journey through the column,but larger substances travel relatively rapidly through the column
SUMMARY Gel filtration chromatography uses columns filled with porous resins that let in smaller substances, but exclude larger ones. Thus, the smaller substances are slowed in their journey through the column, but larger substances travel relatively rapidly through the column
(a) Electrophoresis. -origin 5.2 Labeled Tracers(示踪标记) migration Autoradiography(放射自显影) Radioactive DNA is a means of detecting radioactive fragments compounds with a photographic emulsion(感光乳剂)/x-ray film. Place next to x-ray film (b)Autoradiography. By measuring the area under each peak one can get an estimate of the radioactivity in each band expose A densitometer（显像密度计) passes a beam of light Light through an autoradiograph absorbance Develop film and measures the absorbance of that light by the sample. Distance from origin dark bands correspond to Autoradiograph: the DNA bands on the gel 9 Densitometry. Figure 5.8 Autoradiography
Radioactive DNA fragments 5.2 Labeled Tracers(示踪标记) Autoradiography（放射自显影） is a means of detecting radioactive compounds with a photographic emulsion（感光乳剂）/ x-ray film . expose dark bands correspond to the DNA bands on the gel A densitometer（显像密度计） passes a beam of light through an autoradiograph and measures the absorbance of that light by the sample. By measuring the area under each peak one can get an estimate of the radioactivity in each band