Two-dimensional Gel Electrophoresis First Decreasing High resolution protein separations dimension Isoelectric focusing First step:Isoelectric focusing electrophoresis (FE,等电聚焦电泳)，separating protein based on charge or isoelectric point. gel is placed on SDS polyacrylamide gel. Second step:SDS-PAGE,separating based on size. Seeond dimension SDS polyacrylamide gel electrophoresis Decreasing
Two-dimensional Gel Electrophoresis High resolution protein separations Second step: SDS-PAGE, separating based on size. First step: Isoelectric focusing electrophoresis (IFE,等电聚焦电泳), separating protein based on charge or isoelectric point
Protein Two-dimensional Gel Electrophoresis Sample IEF SDS-PAGE Isoelectric focusing 8 8 +
- + Isoelectric focusing Two-dimensional Gel Electrophoresis
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SUMMARY High-resolution separation of polypeptides can be achieved by two-dimensional gel electrophoresis,which uses isoelectric focusing in the first dimension and SDS-PAGE in the second
SUMMARY High-resolution separation of polypeptides can be achieved by two-dimensional gel electrophoresis, which uses isoelectric focusing in the first dimension and SDS-PAGE in the second
Ion-exchange chromatography(离子交换层析)uses a resin (to separate substances according to their charges. Fractionation of proteins by charge Elution buffer lon-exchang默resin protein mixture Removed the substances that had bound to the resin in (+ the column by passing a solution of gradually increasing beads with K ionic strength positive charge through the column. positively charged protein flows ④⊕④ through column ⊕⊕
Ion-exchange chromatography (离子交换层析) uses a resin （树脂）to separate substances according to their charges. Ion-exchange resin Elution buffer Removed the substances that had bound to the resin in the column by passing a solution of gradually increasing ionic strength through the column