Tumor Antigen Tumor-specific antigen Antigen that are expressed on tumor cells but not on normal cells were called tumor- specific antigens, some of these antigens are unique to individual tumors, whereas others are shared among tumors of the same type
DEPARTMENT OF IMMUNOLOGY Tumor Antigen ❖ Tumor-specific antigen Antigen that are expressed on tumor cells but not on normal cells were called tumor- specific antigens; some of these antigens are unique to individual tumors, whereas others are shared among tumors of the same type
Tumor Antigen Tumor-associated antigen Tumor antigens that are also expressed on normal cellls were called tumor-associated antigens; in most cases, these antigens are normal cellular constituents whose expression is aberrant or dysregulated in tumors
DEPARTMENT OF IMMUNOLOGY Tumor Antigen ❖ Tumor-associated antigen Tumor antigens that are also expressed on normal cells were called tumor-associated antigens; in most cases, these antigens are normal cellular constituents whose expression is aberrant or dysregulated in tumors
Tumor Antigen Generation of tumor-specific CTL clones Melanoma cells from tumor site Isolate sect tumo Coculture mononuclear cells d melanoma cells Tumor Melanoma cell line Identification of tumor antigens recognized by tumor-specific CTLs Lysis of umo CDNA lib cell line Coculture with CTL clone Melanoma cell line Abbas et al: Cellular and Molecular Immunology, Updated 6th Edition pyright9 2009 by Saunders, an imprint of Elsevier, Inc. All rights reserved
DEPARTMENT OF IMMUNOLOGY Tumor Antigen
Tumor Antigen The modern classification is relies on the molecular structure and source of the antigen
DEPARTMENT OF IMMUNOLOGY Tumor Antigen The modern classification is relies on the molecular structure and source of the antigen
Tumor Antigen Examples Normal host cell display Normal multiple M I self protein associated self antigens No T cell response Various mutant proteins in Mutated self carcinogen-or radiation protein nduced animal tumors various mutated proteins in melanomas 2 Product of Oncogene products: mutated oncogene or Ras, Bcr/Abl fusion proteins mutated tumor suppressor Tumor suppressor gene c ao gene products CTL mutated p53 protein Overexpressed: tyrosinase o Overexpressed gp100, MART in melanomas or aberrantly Aberrantly expressed expressed cancer-testis antigens 点 self protein CD8+ (MAGE, BAGE) CTL Human papillomavirus E6, E7 proteins in cervical Oncogenic carcinoma; EBNA protein- virus Virus induced lymphomas antigen specific CD8+ CTL
DEPARTMENT OF IMMUNOLOGY Tumor Antigen