2.2 Genes Individual Individual Total DNA A-fragments B-fragments digested can be are digested are digested with both with enzyme B with enzyme A enzymes mapped by 101ch 导 110mh 205 品 restriction 山 cleavage ↓↓↓↓ Figure 2.2 Double digests define the 90中 90中 90中 cleavage positions 000 0时 000 0g0 of one enzyme with 80 600 regard to the other 2op 500 清菜大当
Figure 2.2 Double digests define the cleavage positions of one enzyme with regard to the other. 2.2 Genes can be mapped by restriction cleavage
2100 A The A-2100 2.2 Genes can 2ene发 1 B-cutting site be mapped by 1900 restriction "2i00 A-digest fragm ents cleavage m mmmmmmmmmmmm mmm Double digest Overlap with the B-2500 traqment Figure 2.3 1900 extendsthe map -"2500 digest fragm ents to the right A restriction map can be "2U而 .→月 constructed by Overlap with B- T亚.中亚.，d 1200 extends m ap relating the A- to lef ovenap wth ←"i00≥ 1900 A-1400 extends ←一1200 map to right fragments and B- "250" fragments through the overlaps seen A Left end of map is with double digest dentified by A- 1000&B-1200 fragm ents,right fragments. 1900 end is identifed by 2500 .B73→ A-500andB-1300 清菜大当
Figure 2.3 A restriction map can be constructed by relating the Afragments and Bfragments through the overlaps seen with double digest fragments. 2.2 Genes can be mapped by restriction cleavage
2.2 Genes can be mapped by restriction cleavage Figure 2.4 When Restriction map restriction fragments are identified by Fragments identified on gel their possession of a A-G (unbroken) labeled end,each A-B-C-D-E-F fragment directly shows the distance BE-B-E of a cutting site A-B-C from the end B Successive A 1re9ien8中crease in length by the distance between adjacent restriction Labeled end sites 情菜大当
Figure 2.4 When restriction fragments are identified by their possession of a labeled end, each fragment directly shows the distance of a cutting site from the end. Successive fragments increase in length by the distance between adjacent restriction sites. 2.2 Genes can be mapped by restriction cleavage
2.2 Genes can be mapped by restriction cleavage AB A B A 1000200 1900 60800500 Figure 2.5 A restriction map is a linear sequence of sites separated by defined distances on DNA.The map identifies the sites cleaved by enzymes A and B.as defined by the individual fragments produced by the single and double digests 清菜大当
Figure 2.5 A restriction map is a linear sequence of sites separated by defined distances on DNA. The map identifies the sites cleaved by enzymes A and B, as defined by the individual fragments produced by the single and double digests. 2.2 Genes can be mapped by restriction cleavage
2.3 How variable are individual genomes? Haplotype is the particular combination of alleles in a defined region of some chromosome.in effect the genotype in miniature.Originally used to described combinations of MHC alleles.it now may be used to describe particular combinations of RFLPs SNP(single nucleotide polymorphism)is any site at which a single nucleotide has changed when two (haploid)genomes are compared 清菜大当
Haplotype is the particular combination of alleles in a defined region of some chromosome, in effect the genotype in miniature. Originally used to described combinations of MHC alleles, it now may be used to describe particular combinations of RFLPs. SNP (single nucleotide polymorphism) is any site at which a single nucleotide has changed when two (haploid) genomes are compared. 2.3 How variable are individual genomes?