© Transformation Donor Free DNA Recipient Transformation is a process by which free DNA is incorporated into a recipient cell and brings about genetic change.转化是游离DNA进入 受体细胞并整合而带来遗传改变的过程。 Chen Feng,Shanghai Jiao Tong University
Chen Feng, Shanghai Jiao Tong University Transformation is a process by which free DNA is incorporated into a recipient cell and brings about genetic change.转化是游离DNA进入 受体细胞并整合而带来遗传改变的过程
Bacterial Transforming DNA chromosome from donor cell Mechanism of DNA-binding protein transformation转化机制 Competence-specific,single-strand DNA-binding protein Recipient cell (a) Binding DNA (a)Binding of free ds Nuclease Free nucleotides DNA by a membrane- bound DNA binding RecA protein protein. Uptake of ssDNA (b)Passage of one of (c) RecA-mediated homologous recombination the two strands into the cell while nuclease degrades the other Transformed recipient cell (d) strand. 2012 Pearson Education.inc
Mechanism of transformation转化机制 (a) Binding of free ds DNA by a membranebound DNA binding protein. (b) Passage of one of the two strands into the cell while nuclease degrades the other strand
Bacterial Transforming DNA chromosome from donor cell DNA-binding protein Competence-specific,single-strand DNA-binding protein Recipient cell (a) Binding DNA (c)The single strand in the cell is bound by Nuclease Free nucleotides specific proteins,and recombination with RecA protein homologous regions of Uptake of ssDNA the bacterial chromosome mediated by RecA protein occurs. (c) RecA-mediated homologous recombination 0 (d)Transformed cell.If recombination does not Transformed recipient cell occur,the incoming (d) 2012 Pearson Educstion inc. DNA cannot replicate and will be lost
(c) The single strand in the cell is bound by specific proteins, and recombination with homologous regions of the bacterial chromosome mediated by RecA protein occurs. (d) Transformed cell. If recombination does not occur, the incoming DNA cannot replicate and will be lost
10.8 Transduction转导 Donor Transduction Virus injection, chromosome disruption DNA-containing viruses Two kinds of transductions: Generalized transduction:DNA from any portion of the host genome becomes a part of the mature virion in place of the virus genome.普遍性转导 Specialized transduction:DNA from a specific region of the host chromosome is integrated directly into the virus genome-usually replacing some of the virus genes.局限性转导
Chen Feng, Shanghai Jiao Tong University 10.8 Transduction 转导 Two kinds of transductions: Generalized transduction: DNA from any portion of the host genome becomes a part of the mature virion in place of the virus genome.普遍性转导 Specialized transduction: DNA from a specific region of the host chromosome is integrated directly into the virus genome- usually replacing some of the virus genes.局限性转导
盒 10.8 Transduction Generalized transduction:DNA derived from virtually any portion of the host genome is packaged inside the mature virion(Figure 10.14) Defective virus particle incorporates fragment of the cell's chromosome randomly Virus can be temperate or virulent ·Low efficiency PLAY Animation:Generalized Transduction Chen Feng,Shanghai Jiao Tong University
Chen Feng, Shanghai Jiao Tong University 10.8 Transduction Generalized transduction: DNA derived from virtually any portion of the host genome is packaged inside the mature virion (Figure 10.14) • Defective virus particle incorporates fragment of the cell’s chromosome randomly • Virus can be temperate or virulent • Low efficiency Animation: Generalized Transduction