一、生物化学及其研究内容 二、生命及其构成 三、生物化学的发展 四、我国古代劳动人民的贡献 五、近代生物化学的发展 六、我国科学家在近代生物化学发展史中的贡献 七、生物化学与其他学科间的关系

《生物化学》课程PPT教学课件：引言——蛋白质概述

第一节 概述 第二节 酵母菌 第三节 霉菌

第一节 病毒的形态结构与化学组成 第三节 病毒的主要类群与繁殖方式

Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer

Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?

Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??