I Recombinant DNA technology 1 Restriction enzymes 2 Nucleic acid hybridization °3 DNA cloning ·4 iruses °5 DNA sequencing 6 Polymerase chain reaction
I Recombinant DNA technology • 1 Restriction enzymes • 2 Nucleic acid hybridization • 3 DNA cloning • 4 Viruses • 5 DNA sequencing • 6 Polymerase chain reaction
Restriction enzymes ● Overview o Restriction enzyme digestion ● Nomenclature ● Gel electrophoresis ● Restriction maps O Restriction fragment length polymorphisms(RFLP)
• Overview • Restriction enzyme digestion • Nomenclature • Gel electrophoresis • Restriction maps • Restriction fragment length polymorphisms(RFLP) Restriction enzymes
Overview Restriction enzymes allow dna to be cut at specific sites; Nucleic acid hybridization allows the detection of specific nucleic acid sequences; DNA sequencing can be used to easily determine the nucleotide sequence of a dna molecule
Overview • Restriction enzymes allow DNA to be cut at specific sites; Nucleic acid hybridization allows the detection of specific nucleic acid sequences; DNA sequencing can be used to easily determine the nucleotide sequence of a DNA molecule
Restriction endonuclease (Restriction enzyme) Bacterial enzymes which cut DNA into defined and reproducible fragments Identified in the late 1960s Key discovery which allowed the dNa cloning to become a reality
Restriction endonuclease (Restriction enzyme) • Bacterial enzymes which cut DNA into defined and reproducible fragments • Identified in the late 1960s • Key discovery which allowed the DNA cloning to become a reality
ol, chromosome potB dinB (a RecA exA b repressor Damaged DNA single-strand gap aanc.D CerA Replication porB dinB ZorB ∠A Inactivate 5零 activate Single-stranded DNA RecA complex a42 E