H protein synthesis H1 The genetic code H2 Protein synthesis(translation in prokaryotes H3 Translation in eukaryotes 14 Protein targeting H5 Protein glycosylation
• H1 The genetic code • H2 Protein synthesis(translation) in prokaryotes • H3 Translation in eukaryotes • H4 Protein targeting • H5 Protein glycosylation H protein synthesis
Restriction enzymes ● Overview O Restriction enzyme digestion ● Nomenclature ● Gel electrophoresis ● Restriction maps o Restriction fragment length polymorphisms(RFLP)
• Overview • Restriction enzyme digestion • Nomenclature • Gel electrophoresis • Restriction maps • Restriction fragment length polymorphisms(RFLP) Restriction enzymes
H1 The genetic code The genetic code is a triplet code The genetic code is degenerate Universality of the genetic code ° Reading frames Open reading frames
• The genetic code is a triplet code • The genetic code is degenerate • Universality of the genetic code • Reading frames • Open reading frames H1 The genetic code
Triplet code The nucleotide sequence of the mRNA is colinear with the amino acid sequence of the polypeptide in encodes The relationship between them is called the genetic code 5--3N.C terminal
Triplet code • The nucleotide sequence of the mRNA is colinear with the amino acid sequence of the polypeptide in encodes. The relationship between them is called the genetic code • 5’---3’ N terminal-----C terminal
Triplet code The three nucleotides in group called codons 43=64 codons Initiation codon (AUG) stop codon(UAA, UAG, UGA)
Triplet code • The three nucleotides in group called codons • 4 3=64 codons • Initiation codon (AUG) • stop codon ( UAA, UAG,UGA)