Gel electrophoresis Gel electrophoresis:separates DNA molecules based on size Electrophoresis uses an electrical field to separate charged molecules 。 Gels are usually made of agarose,a polysaccharide 0 Nucleic acids migrate through gel toward the positive electrode due to their negatively charged phosphate groups Gels can be stained with ethidium bromide and DNA can be visualized under UV light Chen Feng,Lecture of Microbiology
Chen Feng, Lecture of Microbiology Gel electrophoresis Gel electrophoresis: separates DNA molecules based on size • Electrophoresis uses an electrical field to separate charged molecules • Gels are usually made of agarose, a polysaccharide • Nucleic acids migrate through gel toward the positive electrode due to their negatively charged phosphate groups Gels can be stained with ethidium bromide and DNA can be visualized under UV light
Figure 11.2 A B D Size in base pairs 5000 4000一 3000一 2000- 1800- 1000一 500- + (a) () 2012 Pearson Education,Inc 2012 Pearson Education,Inc
Figure 11.2 © 2012 Pearson Education, Inc