History American Legion Convention in 1976 in Philadelphia hotel Respiratory distress and fever 200 affected 34 died BIOL 533 Lecture 14
BIOL 533 2 Lecture 14 History • American Legion Convention in 1976 in Philadelphia hotel – Respiratory distress and fever – 200 affected – 34 died
History Six months after outbreak cdc isolated bacteria from post-mortum lung tissue Inoculated lung tissue into peritoneal cavity of guinea pigs After animal became ill, removed its spleen and injected tissue into fertilized chicken eggs (rickettsial methodology) BIOL 533 Lecture 14
BIOL 533 3 Lecture 14 History • Six months after outbreak, CDC isolated bacteria from post-mortum lung tissue – Inoculated lung tissue into peritoneal cavity of guinea pigs – After animal became ill, removed its spleen and injected tissue into fertilized chicken eggs (rickettsial methodology)
History Organism was thought to be unique, given new genus and species name Legionella pneumophila Epidemiological search revealed outbreak in same hotel two years earlier o Culture problems because organisms Do not grow on common laboratory media Do not readily stain BIOL 533 Lecture 14
BIOL 533 4 Lecture 14 History • Organism was thought to be unique, given new genus and species name: Legionella pneumophila – Epidemiological search revealed outbreak in same hotel two years earlier • Culture problems because organisms: – Do not grow on common laboratory media – Do not readily stain
Microbial Physiology and structure Family Legionellaceae One genus: Legione∥a 25 species and 42 serotypes Legionella pneumophila responsible for 85% of infections(serotype 1 most common) BIOL 533 Lecture 14
BIOL 533 5 Lecture 14 Microbial Physiology and Structure • Family Legionellaceae • One genus: Legionella – 25 species and 42 serotypes – Legionella pneumophila responsible for 85% of infections (serotype 1 most common)
Microbial Physiology and structure Species can be differentiated by DNA homology Cell wall fatty acids Biochemical testing Immunological serotyping BIOL 533 Lecture 14
BIOL 533 6 Lecture 14 Microbial Physiology and Structure • Species can be differentiated by: – DNA homology – Cell wall fatty acids – Biochemical testing – Immunological serotyping