Microbial Physiology and structure Morphology Gram rods(pleomorphic on artificial media) Do not stain well except with special silver stain Culture: do not grow in ordinary lab media, even though they are aerobic organisms Require high concentration of cysteine and are inhibited by sodium ions and aromatic compounds BIOL 533 7 Lecture 14
BIOL 533 7 Lecture 14 Microbial Physiology and Structure • Morphology – Gram— rods (pleomorphic on artificial media) • Do not stain well except with special silver stain – Culture: do not grow in ordinary lab media, even though they are aerobic organisms • Require high concentration of cysteine and are inhibited by sodium ions and aromatic compounds
Microbial Physiology and structure Media developed have Charcoal to absorb aromatic compounds Non-sodium ion buffer Antibiotics to suppress other organisms Dyes to make colonies visible on solid media BIOL 533 Lecture 14
BIOL 533 8 Lecture 14 Microbial Physiology and Structure • Media developed have: – Charcoal to absorb aromatic compounds – Non-sodium ion buffer – Antibiotics to suppress other organisms – Dyes to make colonies visible on solid media
Pathogenesis Encounter: aquatic, found in lakes and streams Large numbers in polluted water around power stations Can live in chlorinated drinking water Normally found in hot water tanks of buildings Get into pipes and multiply in sediment and accumulate over period of years Do not spread from person to person Sediment provides shelter as well as nutrition for other BIOL 533 bacteria that can supply cysteine Lecture 14
BIOL 533 9 Lecture 14 Pathogenesis • Encounter: aquatic; found in lakes and streams – Large numbers in polluted water around power stations – Can live in chlorinated drinking water – Normally found in hot water tanks of buildings • Get into pipes and multiply in sediment and accumulate over period of years – Do not spread from person to person – Sediment provides shelter as well as nutrition for other bacteria that can supply cysteine
Pathogenesis Entry, spread, and multiplication Entry: inhalation of organisms Incubation period: 2-10 days Initial host response: acute inflammatory response of aveoli and then bronchioles (similar to pneumococcal infection) Neutrophils accumulate followed by macrophage BIOL 533 Lecture 14
BIOL 533 10 Lecture 14 Pathogenesis • Entry, spread, and multiplication – Entry: inhalation of organisms • Incubation period: 2-10 days – Initial host response: acute inflammatory response of aveoli and then bronchioles (similar to pneumococcal infection) • Neutrophils accumulate followed by macrophage