Choosing an Animal model Pathogen may not affect animal at all OR may give different symptoms Given disease may have a number of animal models none of which fully satisfies characteristics of disease BIOL 533 Lecture three
BIOL 533 2 Lecture Three Choosing an Animal Model • Pathogen may not affect animal at all -ORmay give different symptoms • Given disease may have a number of animal models, none of which fully satisfies characteristics of disease
Choosing an Animal model ep One model may show certain aspects of disease, but not another Different models may rely on different routes of introducing pathogen; e. g Bordetella pertussis ● Intracrania ● Interperitoneal Respiratory aspiration BIOL 533 Lecture three
BIOL 533 3 Lecture Three Choosing an Animal Model • One model may show certain aspects of disease, but not another • Different models may rely on different routes of introducing pathogen; e.g.: – Bordetella pertussis • Intracranial • Interperitoneal • Respiratory aspiration
Choosing an Animal model Ideally, want model to Use same route as human disease Display same symptoms Display same virulence Alternative: cell culture, organ culture BIOL 533 Lecture three
BIOL 533 4 Lecture Three Choosing an Animal Model • Ideally, want model to: – Use same route as human disease – Display same symptoms – Display same virulence • Alternative: cell culture, organ culture
Cell Culture/Organ Culture e Difficult Cell-lines often tumor lines that are genetically and physiologically different (immortal--many mutations) Removed from effects of other organs hormones · Cells grown in artificial media differ from∥Wivo Cell lines may not express same Ag on surface as When in animal BIOL 533 Lecture three
BIOL 533 5 Lecture Three Cell Culture/Organ Culture • Difficult • Cell-lines often tumor lines that are genetically and physiologically different (immortal—many mutations) • Removed from effects of other organs, hormones • Cells grown in artificial media differ from in vivo • Cell lines may not express same Ag on surface as when in animal
Studying Pathogenic Organisms a5 Look at phylogeny to find closely related organisms, for example S, typhimurium vs One may respond more easily than the other to variety of genetic techniques BIOL 533 Lecture three
BIOL 533 6 Lecture Three Studying Pathogenic Organisms • Look at phylogeny to find closely related organisms; for example: – S. typhimurium vs. – S. typhi One may respond more easily than the other to variety of genetic techniques