《遗传学》课程教学资源（PPT课件，英文版）Is there any similarity between human and model organisms?

Is there any similarity between human and model organisms?

Is there any similarity between human and model organisms?

Human development

Human development

Drosophila embryos

Drosophila embryos

Using functional conservation to clone human genes Yeast with mutation Expose to human CDNA library Permissive growth conditions Human DNA (different human DNA in each cell) Restrictive Growth conditions Dies Dies Dies Only this cell grows Conclusion: Human DNA contains gene that was mutant(disfunctional) in yeast DNA

Using functional conservation to clone human genes

Using mutants to analyze the pathway or functions of genes Mutants have to be generated to analyze the pathway or functions of genes Saturation screening Overexpression Suppressor mutations Synthetic lethals Nonallelic noncomplementation

Using mutants to analyze the pathway or functions of genes • Mutants have to be generated to analyze the pathway or functions of genes • Saturation screening • Overexpression • Suppressor mutations • Synthetic lethals • Nonallelic noncomplementation