Nucleotides and nucleic Acids(continued) These slides are pre-release and the real lecture slides might be slightly modified to improve the visual effect
Nucleotides and Nucleic Acids (continued) These slides are pre-release and the real lecture slides might be slightly modified to improve the visual effect
Post double-Helix era
Post Double-Helix Era
1957 Arthur Kornberg (1918-)of the U.S. produced DNa in a test tube. 1963 F. Sanger(1918-)of Britain developed sequencing procedure for proteins and later for dna
1957 Arthur Kornberg (1918- ) of the U.S. produced DNA in a test tube. 1963 F. Sanger (1918- ) of Britain developed sequencing procedure for proteins, and later for DNA
Sanger Sequencing Method 0 BASE dideoxy nucleotide DHA AATCTGGGCTACTCGGGCG DNA POLYMERASE 4 dNTP LABELED ddATP PRIMER TGAG C G C G G AGCC 16 18 AGACCCGATGAGC G C G
Sanger Sequencing Method
DHA 3 AATCTGGGCTACTCGGGCG CGCA DNA POLYMERASE 十4dNTP ddatp ddttp ddctp ddGTP TAGACCCGATGAGcCCGC