NH2 R 5.2 Transfer RNA C-H Amino acid Amino acid linked 0 to 3 end of tRNA is the adapter 0 CH2 Figure 5.2 A tRNA has the Amino dual properties of an adaptor that recognizes 51 both the amino acid and Arm consists of codon.The 3 adenosine Base-paired stem is covalently linked to an IIIr农 amino acid.The anticodon base pairs with the codon Single- on mRNA. stranded loop 2 Anticodon- codon pairing 清苇大当 NNNNNNN mRNA
Figure 5.2 A tRNA has the dual properties of an adaptor that recognizes both the amino acid and codon. The 3 adenosine is covalently linked to an amino acid. The anticodon base pairs with the codon on mRNA. 5.2 Transfer RNA is the adapter
5.2 Transfer RNA is the adapter Acceptor arm D am 23456 ACC13210知85 TyC arm U*7 Py59 Figure 5.3 The tRNA 16Pu 1 9 65646362 1312Py1 95051520 Pu G cloverleaf has G208 2223Pu2 Py 2 7 invariant and semi- 27.49444546 28- invariant bases,and a 30 40 Extra arm conserved set of base 38 pairing interactions. 56r Anticodon 情菜大当
Figure 5.3 The tRNA cloverleaf has invariant and semiinvariant bases, and a conserved set of base pairing interactions. 5.2 Transfer RNA is the adapter
Clover leaf secondary structure has four arms 3 5.2 Transfer RNA Amino acid stem D arm Tyc arm is the adapter Anticodon arm 2D projection has 2 perpendicular duplex regions Figure 5.4 Transfer RNA folds into a compact L-shaped Backbone follows L-shaped structure tertiary structure with Amino acid the amino acid at one NH end and the anticodon at Tyc arm Amino acid stem the other end D am Anticodon arm 清苇大当 Anticodon
Figure 5.4 Transfer RNA folds into a compact L-shaped tertiary structure with the amino acid at one end and the anticodon at the other end. 5.2 Transfer RNA is the adapter
5.2 Transfer RNA is the adapter Figure 5.5 A space-filling model shows that tRNAPhe tertiary structure is compact.The two views of tRNA are rotated by 900 Photograph kindly provided by S.H.Kim 情華大当
Figure 5.5 A space-filling model shows that tRNAPhe tertiary structure is compact. The two views of tRNA are rotated by 90o . Photograph kindly provided by S. H. Kim. 5.2 Transfer RNA is the adapter
5.2 Transfer RNA is the adapter SH D.esulfuration CH,Cysteine changes CHa Alanine amino acid MH NH2 68 Codon recognition unchanged 06& Figure 5.6 The meaning of tRNA is determined by its anticodon and not by its amino acid 清苇大当
Figure 5.6 The meaning of tRNA is determined by its anticodon and not by its amino acid. 5.2 Transfer RNA is the adapter