Wireg Chen'4 Group of Bioinformatics @College of Life Science,Zhejiang University 0心转录组学000队 01/15,2017 ventson@zju.edu.cn
转录组学 01/15, 2017 ventson@zju.edu.cn Ming Chen’s Group of Bioinformatics @College of Life Science, Zhejiang University
Ting Chen'4 Group of Bioinformatics @College of Life Science,Zhejiang University 00八 前言 replication (DNA->DNA) DNA Polymerase 基因组学 QD0①d1 DNA transcription (DNA->RNA) RNA Polymerase RNA 转录组学 translation (RNA->Protein) Ribosome ○OOoO-Protein 蛋白质组学 from en.wikipedia 中心法则：遗传信息传递 2
前言 Ming Chen’s Group of Bioinformatics @College of Life Science, Zhejiang University from en.wikipedia 基因组学 转录组学 蛋白质组学 中心法则：遗传信息传递 2
Zig Che'4 Group of Bioinformatics @College of Life Science,Zhejiang University 00心 技术发展 国日 Experiment-based Hybridization-based Sequencing-based Advanced seq ·Northern blot ·Microarray ·SAGE ·NGS ·RT-PCR ·CAGE ·3GS ·MPSS ·Single cell 转录组学研究技术革新 3
技术发展 Ming Chen’s Group of Bioinformatics @College of Life Science, Zhejiang University Experiment-based • Northern blot • RT-PCR Hybridization-based • Microarray Sequencing-based • SAGE • CAGE • MPSS Advanced seq • NGS • 3GS • Single cell 转录组学研究技术革新 3
ing Chen'4 Group of Bioinformatics @College of Life Science,Zhejiang University 00八应用 0.5- CP 盖 0.3 Neurons 02 RGs 0.1 差异表达 可变剪切 共表达 转录调控 4
应用 Ming Chen’s Group of Bioinformatics @College of Life Science, Zhejiang University 差异表达 可变剪切 共表达 转录调控 4
WigChe'4 Group of Bioinformatics @College of Life Science,Zhejiang University O价RNA测序(RNA--sequencing) AARAAAA西 TTTI TGATAGATC Reverse transcriptase GGCGATCG TAGCTGTAC ARAAAAAA TTTT RNA CDNA Library Data isolation amplification Sequencing preparation analysis from GATC Biotech DNA 1.试验设计 2.测序流程 3.数据分析 4.验证实验 5
RNA测序（RNA-sequencing） Ming Chen’s Group of Bioinformatics @College of Life Science, Zhejiang University 1.试验设计 2.测序流程 3.数据分析 4.验证实验 from GATC Biotech 5