HBeAg is the product of Pre and c gene HBV C gene Pre-C region region Expression 29AA 181AA Pre C/ C Protein HBeAg HBCAG g 19AAI 34AA HBeAg present in the serum
HBeAg is the product of Pre and C gene HBV C gene Pre-C region C region Pre C / C Protein HBeAg HBcAg Expression Secreted outside of the cells HBeAg present in the serum 29AA 181AA --19AA ---34AA
PreC Mutation in the Pre C region HBeAg result in no synthesis of HBeAg HBcAg区 and HBv patients without HBeAg (-) thus appears---HBeAg(-) patients
PreC C HBcAg 区 HBeAg Mutation in the Pre C region result in no synthesis of HBeAg， and HBV patients without HBeAg （-），thus appears---HBeAg(-) patients
preCI C S1 S TP SPACER RT RNaseh ▲▲▲▲▲ 178 347 691 E 421~436505~528546~556576~589592~600 Functional domains of HBV polymerase boo)eca2)1182-33
TP SPACER RT preC C S1 S2 S X RNaseH 1 178 347 691 843 421~436 505~528 546~556 576~589 592~600 652 A B C D E preC C S1 S2 S X SPACER RNaseH Functional domains of HBV polymerase J Virol. 2001 Dec;75(23):11827-33. 1
Hepatitis b virus mutants a Variations in nucleotide sequence in one of the HBV genes can result in change in the virological and, in some cases, clinical features of the infection aS gene: vaccine or HBIG escape mutants C gene: can affect disease severity or serological and clinical manifestations I P gene; can effect replicative efficiency and resistance to antiviral therapy
Hepatitis B Virus Mutants Variations in nucleotide sequence in one of the HBV genes can result in change in the virological and, in some cases, clinical features of the infection. S gene: vaccine or HBIG escape mutants C gene: can affect disease severity or serological and clinical manifestations P gene: can effect replicative efficiency and resistance to antiviral therapy
2、HBVv: Replication Reverse transcription: one of the mrNAs is replicated with a reverse transcriptase making the dNa that will eventually be the core of the progeny virion RNA intermediate: HBV replicates through an RNA intermediate and produces and release antigenic decoy particles Integration: Some DNA integrates into host genome causing carrier state
2、HBV: Replication • Reverse transcription: one of the mRNAs is replicated with a reverse transcriptase making the DNA that will eventually be the core of the progeny virion • RNA intermediate: HBV replicates through an RNA intermediate and produces and release antigenic decoy particles. • Integration: Some DNA integrates into host genome causing carrier state