pre-S2 pre-S1 GRE mzOm 〔- strand,24kb [+]strand, 3. 4kb TATAA U5-like DR1 Enhancer DR2 pre
GENOME
Open Reading frames There are 4 open reading frames derived from the same strand(the incomplete strand) S-the 3 polypeptides of the sur face antigen(presl, preS2 and S-produced from alternative translation start sites ·C- the core protein P-the polymerase X-a transactivator of viral transcription(and cellular genes?) HBx is conserved in all mammalian(but not avian) hepadnaviruses. Though not essential in transfected cells, it is required for intection In vIvo
There are 4 open reading frames derived from the same strand (the incomplete + strand) • S - the 3 polypeptides of the surface antigen (preS1, preS2 and S - produced from alternative translation start sites. • C - the core protein • P - the polymerase • X - a transactivator of viral transcription (and cellular genes?). HBx is conserved in all mammalian (but not avian) hepadnaviruses. Though not essential in transfected cells, it is required for infection in vivo. Open Reading Frames
H B GEnome 2.1kb RNA 2. 4kb RNA Pre-s1 Pre-S2 -strand ORF-S +strand 3.5kb RNA DR1 ORF-P DR2 Pre-C ORF-X 0.7kb RNA
A A A A A A A A A A A A 2.1kb RNA 2.4kb RNA 3.5kb RNA 0.7kb RNA Pre-S1 Pre-S2 ORF-S ORF-P ORF-X ORF-C DR1 5’ 5’ +strand -strand Pre-C DR2 ＨＢＶGenome
The s gene has three start codons and is capable of producing three different sizes of HBSAg(small, medium, and large s) The C gene has two start codons and can produce two antigenically distinct products: the HBCAg retained in hepatocytes until assembled as core particles HBeAg secreted into the serum as a small soluble protein
19 • The S gene has three start codons and is capable of producing three different sizes of HBsAg (small, medium, and large S). • • The C gene has two start codons and can produce two antigenically distinct products: • the HBcAg retained in hepatocytes until assembled as core particles. • HBeAg secreted into the serum as a small soluble protein
PreS2 SHBs 55AA MHBS 119AA LHBS 400AA 281AA 226AA Ⅴ iral envelope Large hBs protein pre SI (Virion) proteins esp LHBS play a pivotal role in Extracellular HBV entry; NE > My >Pres1(21-47)is the cec putative viral Cytoplasm attachment site to hepatocytes
Viral envelope proteins esp. LHBs play a pivotal role in HBV entry; PreS1(21-47) is the putative viral attachment site to hepatocytes. PreS1 PreS2 S ATG ATG ATG TAG SP1 SP2 LHBs MYR 1 119 174 400 SHBs MHBs 400AA 281AA 226AA 55AA 119AA