HCBI Sequence iever v2.0- icrosoft Internet Explorer 件)编辑啁)查看)收藏()工具〔)帮助p ⊙·因图P次收,号回·回的供器 链接 地址①)htp/wacb. n. nih. gov/entrez/vie181812801b /function="Member of the VHIID regulatory family. Contain omopo l ymeric serine and threonine residues, a putative nuclear localization signal, leucine heptad repeats, and an LXXLL motif. Putative transcriptional regulator repressing the gibberellin response. r /ab xref=GEne ID: 814686" 207.1970 /gene=”RGA1 /locus tag="AT2G01570 /go component-nucleus [pmid 9490740] [pmid 11449051] /go function"transcription factor activity [pmid 11118137][pm1d11449051]" go process="gibberellic acid mediated signaling [pmid 11449051]: regulation of transcription [pmid 11118137] note="gibberellin response modulator (RGA1)/ gibberellin-responsive modulator, identical to GB: Y11336 member of scareCrow family /codon start=1 /product="RGAl (REPRESSOR OF GAl-3 1); transcription 士 actor protein id=NP 1782 66.1 / ch xref="GI:15226311 /ab xref=GEne ID: 814686 /trans lation=NMKRDHHQF QGRL SNHGTSSSSSS ISKDKMMMVKKEEDGGGNMDD ELLAVLGYKVRSSEMAEVALKLEQLETMMSNVQEDGLSHLATDTVHYNPSELYSWLDN MLSELNPPPLP ASSNGLDPVLPSPEICGFPASDYDLKVIPGNAIYQFPAIDSSSSSNN ONKRLKSCSSPDSMVTSTSTGTQIGGVIGT TTTTAAGES TRSVILVDSQENG RLVHALMACAEAIOONNL TLAEALVKQIGCLAVSQAGAMRKVATYF AEALARRIYRL SPPONQIDHCLSDTLQMHF YE TCPYLKF AHF TANQAILEAFEGKKRVHVIDFSMQGL UPALMQALALREGGPP TFRL TGIGPPAPDNSDHL HEV GCKLAQLAEAIHVEFEYRGF VANSL ADLD ASMLELRPSDTEAVAVNSVFELHKLL GRPGGIEKVLGVVKQIKPVIFTV EQESNHNGPVFLDRF TESLHYYSTLFDSLEGVPNSQDKVMSEVYLGKQICNLVACEG DRVERHETLSQUGNRFGSSGLAP AHL GSNAFKQASMLLSVFNSGQ LGWHTRPLITTSAUKLSTAAYN 823..2085 /gene="RGA1 /locus tag= standard name=MASC STS15662 r 开始多”可移动避盘〖) 实验四利用核酸 a NCBI Sequence Vi 回 Microsoft PowerP m甲9:40
a NCBI Sequence Viewer v2.0-I ft Internet Explorer 文件)编辑)查看⑩)收藏)工具①)帮助Q ⊙后退·困图的搜索次收藏买的,号圆·口团徽8洛 Hstitoahttp:/www.ncbi.nlmnihgov/entrez/viewer.fcgi7val-=30677937aitemid=18view=gbwithpArTs 工工E PIN UTNOV-ZUUS DEF INITION Arabidopsis thaliana RGAl (REPRESSOR OF GAl-3 1): transcription factor AT2G01570 (RGA1) mRNA, complete cds ACCESS MH126218 REGION:207.1970 VERSION NM126218.2GI:30677937 EYUORDS SOURCE Arabidopsis thaliana (thale cress ORGANISM Arabidopsis thaliana ukar yota: viridiplantae: streptophyta: Embryophyta: Tracheophyta Spermatophyta: Magno liophyta: eudicotyledons: core eudicotyledons rosids: eurosids II: Brassicales: Brassicaceae Arabidopsis COMMENT ROVISIONAL REFSEQ: This record has not yet been subject to final reference sequence was derived from AT2G01570,/ 003071). The rd is NCBI review. This record has been curated by TAIR. This record is On May 13, 2003 this sequence version replaced gi: 18379255 FEATURES Location/ Qualifiers sourc 1..1764 /organism="Arabidopsis thaliana /mol type="mRNA" /ab xref=taxon: 3702 Nr /chr orme /ecotype=columbia gene <1..>176 /locus tag="AT2G01570 /note="synonym: F2I9 19, F2I9 19, REPRESSOR OF GAl-3, REPRESSOR OF GA1-3 1, RGA, RGA1 /function-"Member of the VHIID regulatory family. Contains idues, a putative nuclear localization signal, leucine heptad repeats, an LXXLL motif. Putative transcriptional regulator repressing the gibberellin response /cb xref=Gene ID: 814686 1..1764 RGA1 /locus _ti ag="AT2G01570 /go component=nucleus [pmid 9490740] [pmid 11449051] Ago function-"transcription factor activity [pmid
e NCBI Sequence iever 2.0- icrosoft Internet Explorer 文件①)编辑)查看①收藏()工具①)帮助0D ⊙后退·困图的搜索次收藏买的,号圆·口团徽8洛 Httitdahttp://www.nebi.nlmnih.gov/entrez/viewer.fcgi?val=30677937&itemid=1aview=gbwithpart ATTATATAGCICGA NCBI S Nucleotide MNc豆 [Sign Inl [Register Structure Taxonomy Search Nucleoti Preview/ndex History Clipboard Details DisplayFASTA 岛w5Fil文件下载 Projection on the feature(#1) Show whole Sequel A区noNA[ Refresh 口上1M126218. Reports Arabidopsis th,g获取文件·相 Www.ncbinlm.nih.gov Comment Features LOCUS NM126218 176估计剩余时文件下载 DEFINITIoN Arabidopsis thaliana RGA1(下载到 factor AT2G01570(RGA1)mN传输速度 您想保存此文件吗 ACCESSIoN NM 126218 REGION: 207..1970 NM126218.2GI:30677937 回下载完毕 ERSION 名称 KE YUORDS 类型:未知文件类型 ORGANISM Arabidopsis thaliana ( Arabidopsis thaliana (thale 发送者:ww,ncbi.nlm, nih.gov Eukar yota: viridiplantae: Streptophyt Spermatophyta: Magnoliophyta; eudicot 保存()取消 rosids: eurosids Ii: Brassicales Br COMMENT PROVISIONAL REFSEQ: This record has NCBI review. This record has been cur derived from an annotated genomic sec 星备是果卷数:果楼受 reference sequence was derived from On May 13, 2003 this sequence versio FEATURES Location/ Qualifiers source organism-Arabidopsis thaliana" /mol type="mRNA" /cb xref=taxon: 3702 /chr
202利用 Unigene数据库进行查找 后,因团批收来的区 地址0)ht:/www.ncbi.nlm.nih.gov/last NCBI Latest news: 6 December 2005: BLAST 2.2. 13 releas BLAST The Basic Local Alignment Search Tool( BLAST)finds regions of local similarity between sequences The program compares nucleotide or protein sequences to sequence databases and calculates the ● Gettin statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships started between sequences as well as help identify members of gene families ●News FAQ Nucleotide Protein Quickly search for highly similar sequences Protein-protein BLAST(blastp Position-specific iterated and pattern- hit ●NAR2004 Quickly search for divergent sequences initiated BLAST(PSI and PHI-BLAST) (discontiguous megablast) Search for short, nearly exact matches Nucleotide-nucleotide BLAST(blastn) Search the conserved domain database Search for short, nearly exact matches (rpsblast) Similarity Search trace archives with megablast or Protein homology by domain architecture Scores discontiguous megablast (cdart) Software Translated Genomes ● Downloads Translated query vs. protein database Human, mouse, rat, chimp, cow, pig, dog, blastx) sheep, cat Other resources Protein query vs, translated database Fly, honey bee, other insects References Translated query vs, translated database Microbes, environmental samples (tblastx) ntributors Plants, nematode Fungi, protozoa, other eukaryotes special Meta Search for gene expression data(GEO Retrieve results BLAST) 开始多0”[数学 实验四利用核 NCBI BLAST - Mic
2.利用Unigene数据库进行查找
HCBI Blast icrosoft Internet Explorer 件①)编辑)查看)收藏()工具①)帮助Qp 因GP☆收来的,圆·回假码器 链接 地址0)htp/wacb. am. nih. gov/b1s(1aa2Cmwm:A0Ars65amAN转到 NCBI nucleotide-nucleotide BLAST Translations Retrieve results for an RI 输入RGA1的 cDS序列 1681 agtaatggat gtttgatgtt gggttggcac actcgtccac Search tcattaccac ctccgcttgg 1741 aaactctcga cggcggcgta ctga Set subsequence from 选择数据库 Choose database est 三ST Now. BLASTd or Reset quan Resoram Options for advanced blastin mit by entrez or select fr( All organisms Choose flter E Low complexity Human repeats Mask for lookup table only [mask lower case Word Size 11v 开始。”「一可移动避盘、「四实验四和、「豇肌t国mct「Cs m收甲9:44
输入RGA1的 CDS序列 选择数据库 “EST