发现基因的一般过程 基因区域预测和基因功能预测2个层 ①寻找DNA序列中基因不可能出现的区域,并 将此遮蔽起来; ②在启动子区寻找一致的模式,找出转录因子识 别DNA结合区域; ③寻找转录的起始密码、终止密码和剪切位点; ④找出编码区
发现基因的一般过程 基因区域预测和基因功能预测2个层次 ①寻找DNA序列中基因不可能出现的区域，并 将此遮蔽起来； ②在启动子区寻找一致的模式，找出转录因子识 别DNA结合区域； ③寻找转录的起始密码、终止密码和剪切位点； ④找出编码区
第一步:获取DNA目标序列 ①如果你已有目标序列,可直接进入第2 步 ②可通过 Pubmed查找你感兴趣的资料 通过 GenBank或MBL等数据库查找 目标序列
第一步：获取DNA目标序列 ① 如果你已有目标序列，可直接进入第2 步； ② 可通过PubMed查找你感兴趣的资料； 通过GenBank或EMBL等数据库查找 目标序列
例:研究拟南芥GRAS基因家 族中 DELLA亚家族
例：研究拟南芥GRAS基因家 族中DELLA亚家族
a Entrez cross-database search- icrosoft Internet Explorer 1。首先对已知GRAs家族中个亚家族 DELLA的基因检索 地址①)萄htt: www, ncbi,nlm, nih. gov query/ query,fcai NCBI C b- Entrez, The Life Sciences Search Engine HOMESEARCH SITE MAP PubMed All Databases Human Genome GenBank Map viewe BLAST Search across databases Welcome to the Entrez cross-database search page M PubMed: biomedical literature citations and abstracts 2B Books: online books PubMed Central: free, full text journal articles 大 OMIM: online Mendelian Inheritance in Man w site Search: NCBI web and FTP sites B O OMIA: online Mendelian Inheritance in Animals Nucleotide: sequence database(Gen Bank) UniGene: gene-oriented clusters of transcript sequences 2 ... Protein: sequence database CDD: conserved protein domain database 出 Genome: whole genome sequences 3D Domains: domains from Entrez Structure 子 o Taxonomy: organisms in GenBank PopSet: population study data sets lin SNP: single nucleotide polymorphism Gene: gene-centered information GEO DataSets: experimental sets of GEo data #+ HomoloGene: eukaryotic homology groups Ei cancer Chromosomes: cytogenetic databases Pubchem Compound: unique small molecule chemical Pubchem BioAssay: bioactivity screens of chemical C Pubrdhsem Substance: deposited chemical substance a M systeA: gene expression atlas of mouse central nervous a
1. 首先对已知GRAS家族中个亚家族DELLA的基因检索
Gene- icrosoft Internet Explorer 文件)编辑吧)查看收藏)工具①)帮助⑩ ⊙后·国图的P数次收来创,品圆·口⑧洛 链接 地址①)@htt:/ wwww ncbi,nla, nih. gov/ entre?/ query,fei?l=gene 习 NCBI tre My NC ene Sign In [Register All Database Genome SearchGene Limits Clipboard Details Entrez Gene is a searchable database of genes, from Refseq genomes, and defined by sequence and or located in the NCBI Map viewer Entrez Gene Home Sample Sear ches About Find genes by Help free text Search Toolbar human muscular dystrophy Gene handbook partial name and multiple species transporterltitlel AND (Drosophila melanogaster orgnl oR"Mus musculus forni) Statistics Downloads chromosome and symbol (Ichrl oR 2[chrl) AND adh*sym (FTP) associated sequence accession number M11313faccnl gene name(symbol) BRCAlsYmI Mailing Lists publication(PubMed ID 11331580PMID Gene Ontology (Go) terms or identifiers Gene 1720G1 Refer chromosome and species YICHRl AND human[oRGNI Enzyme Commission(EC) numbers 19,3,1Ec1 Feedback more ways to search,. Help Desk Corrections About Entrez Gene Corrections· Additions· Feedback About generIfs o Entrez Gene Help o Report a new gene o Frequently Asked Questions o Report a new splice variant Related Sites o Entrez Gene: gene-centered information at NCBI. o How to add information about function (GeneRIF) BLAST e Correct or update a gene record Entrez Genome o General help on the Entrez search and retrieval system o Correct or update a reference sequence Genome Projects o NCBI Handbook Chapter on Entrez Gene (download PDF o Report a publication or GeneRIF error o Download data via FTP