Facultatively Aerobic Gram-Negative Rods H.C-C-cOo vate thMTPPH3C-C-TPP H C-C-Co0 ale acetolactate CH Butanediol-Producing, peritrichous Enteric Bacteria Erwinia carotovora, and its biochemical pathway for formation of butanediol from two CH, molecules of pyruvate H-C-OH 3-Butanediol
Facultatively Aerobic Gram-Negative Rods Butanediol-Producing, peritrichous Enteric Bacteria Erwinia carotovora, and its biochemical pathway for formation of butanediol from two molecules of pyruvate
(a)Mixed acid fermentation(for example, Escherichia coll) Typical products(molar amounts) Glucose yruvate 一 Lactate Acidic. neutral 4:1 cO c2:H2 1:1 Succinate Ethanol Acetyl-CoA Fermentation products Acetate are the key to separate cO2 the enteric bacteria Formate (b) Butanediol fermentation (for example, Enterobacter Typical products(molar amounts 2.3-Butanediol+ co idic. neutra 1:6 Ethanol cO2: H2 5:1 Lactate Glucose pyruvate Succinate Acetate CO2+ H2
Fermentation products are the key to separate the enteric bacteria
Two Broad Patterns of Fermentations Mixed-Acid Fermenters: Butanediol Producers Proteus Klebsiella Citrobacter Enterobacter Edwardsiella Serratia Salmonella Erwinia Escherichia Hafnia shigella
Two Broad Patterns of Fermentations Mixed-Acid Fermenters: Proteus Citrobacter Edwardsiella Salmonella Escherichia Shigella Butanediol Producers Klebsiella Enterobacter Serratia Erwinia Hafnia
Diagnostic test Go to number 1 MR + VP Facultatively (mixed-acid fermenters) MR VP+ Aerobic gram (butanediol producers) 2 Urease 27P346 Proteus Negative Rods Urease 3 H2s (Si+ H2s (si) A simplified key to identify 4 KCN+ KCN Citrobacter the main genera of enteric 5 5 Indole + citrate Edwardsiella bacteria Indole-: citrate+ Salmonella 6 Gas from glucose Escherichia No gas from glucose Shigella 7 Nonmotile: ornithine Klebsiella Motile: ornithine+ 8 8 Gelatin + DNAse Serratia (red pigment Gelatin slow: DNAse Enterobacter K Mixed-acid fermenters Butanediol producers
Facultatively Aerobic GramNegative Rods A simplified key to identify the main genera of enteric bacteria
Escherichia Universal in habitants of the intestinal tract Play nutritional role(synthesizing vitamins) Consume O2, render the large intestine anoxic Some are pathogenic Diarrhea Children’ s nurseries Urinary infections Enterotoxin
Escherichia Universal inhabitants of the intestinal tract Play nutritional role (synthesizing vitamins) Consume O2, render the large intestine anoxic Some are pathogenic Diarrhea Children’s nurseries Urinary infections Enterotoxin