Using dense linkage maps Assigning chromosomal positions: A top-down approach Use M1 as se M2 as hybridization hybridization ()Data generated by screening a ro be to screen probe to screen AC library with seven polymorphic AC library. AC library. markers M1(marker 1) M2(marker 2) Marker loci Portion of chromosome )1 M1 M2 M3M4 M5 M6 M7 clone yM1 Clone vM2 2. Do yM1 and y M2 overlap? Contic Gap Contig Isolate end Clone M1 fragment of y!10 Scale(mb) Restriction Label and use di gestion, and Result of as probe of yM2. electrophoresis hybridization (c)Complete contig spanning region of m2 between Mi and M7 Hybridization demonstrates presence of yM1 M1 M2 M3 M4 M5 M6 M7 end somewhere within clone yM2
(u) Digest chromosomes with restriction enzyme Clone fragments into a genomic cosmid brary Alternative Way to“产 h cosmid for the pattern of restriction Create a Physical 4+5,3-4254 53356 Map: Fingerprinting Cione t Cine 2 Fragment lengths 3.5 (c) Feed fingerprint data into computer programmed to look (d) Arrange overlapping cosmid inserts into 24 chromosomal contigs Clone 3 5,336,4+3+344 5,3s23 Clone 2 上cong
Alternative Way to Create a Physical Map: Fingerprinting