Karyotype: Banding that Characterizes Chromosmes 2 3 101112 首的沿吕x￥首 13141516171819202122Y
Karyotype: Banding that Characterizes Chromosmes
212 211 据 Banding at 12 11.1 Different 1121 1121 1122 112 Resolution 1123 2111 21.12 212 213 213 22 222 223 311 312 312 3131 出3 35 35 35 363
Banding at Different Resolution
The FISH protocol Fluorescent probes Fluorescent e Drop cells onto a 2. gently denature dna by 3. Add hybridization glass slide. treating briefly with DNase. probes labeled with fluorescent dye and Fluorescence microscope wash away unhybridized probe Barier filter 1(blocks dangerous Eyepiece short UV rays, allows needed long Barrier filter 2(further UV rays to pass through) blockage of stray Uv rays) UV source Mirror to UV light transparent to visible light objective lens Object 4. Expose to ultraviolet Uv) light. Take picture of fluorescent chromosomes
Physical map of a chromosome a)Identify an ordered series of overlapping genomic clones Chromos。mec Overlapping clones b)Analyze each clone for restriction sites and gene locations. (Restriction sites) A BC DE FG Genes) (c)Create maps of overlapping genomic clones. 2 3 A BC DE FG d) combine information into a single continuous physical map that spans the length of the chromosome. 山1二 Contig