BIOCHEMISTRYANDMOLECULARBIOLOGYmRNA----Templatefor protein synthesisCharacteristics ofcodon:>universality: The specificity of the genetic code has beenconserved from very early stages of evolution, with only slightdifferences in the manner the code is translated.>Specificity: The genetic code is specific- that is, a specificcodon always codes for the same amino acid.>Wobble: The third base of Codon with the first base ofanticodon is not completely obey base pairingDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Characteristics of codon： ➢universality：The specificity of the genetic code has been conserved from very early stages of evolution, with only slight differences in the manner the code is translated. ➢Specificity：The genetic code is specific- that is, a specific codon always codes for the same amino acid. ➢Wobble：The third base of Codon with the first base of anticodon is not completely obey base pairing. mRNA-Template for protein synthesis
ABIOCHEMISTRYANDMOLECULARBIOLOGY3'Wobble5'tRNAmRNA5'3ATCDepartment of Biochemistryand Molecular Biology,MedicineSchool
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Wobble
GItRNAAnticodonInosineCCmRNAGGUGGCGGACodonInosine(I)can wobblepairwithU,CorA
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY
BIOCHEMISTRYANDMOLECULARBIOLOGYmRNA----Template for protein synthesisCodon and anticodon pairing with wobbletRNAanticodonGCAUwith the firstbasemRNA codonGUA, GU, C,AU. Cwith the thirdbaseDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Codon and anticodon pairing with wobble tRNA anticodon with the first base I U G A C mRNA codon with the third base U, C, A A, G U, C U G mRNA-Template for protein synthesis
Aframeshift mutationinserts anew basepair here...Normal DNA..ATGAACCTTTGATCTAAAGATGACCTTTGATCTAAAGDNATACTGGAAACTAGATTTCTACTTGGAAACTAGATTTCILEEe0tARLAIC...e..ETemplate....strand-..ETranscriptionCELR...E.--.ReadingframesmRNAAUGACCUUUGAUCUAAAGAUGAACCUUUGAUCUAAAGFEREELEERERERECECE.CEStartRESEEcodonL..andnewreadingframe..TranslationEE.ensuesintranslation......ProteinPheMetThrASDMetAsnLeuLeuLysStopFrameshiftsoftenleadtoprematuretermination
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY