H+H+HHH+COS8
O 2 O 2 O 2 O 2 O 2 O 2 H + H + H + + H + H CO 2 CO 2 CO 2 CO 2H + CO 2 O 2 O 2 O 2
XFAnalyzers Measure the Two MajorEnergyPathwaysoftheCellGlucoseH福ATPGlycolysisRespirationMitochondrialATPGlycolysisPyruvateRespiration..................Lactate................Measured bytheMeasuredbytheExtracellularOxygenAcidification RateConsumption Rate(ECAR)(OCR)inFattyAcids3AminoAcidsMk@
XF Analyzers Measure the Two Major Energy Pathways of the Cell Mitochondrial Respiration . Measured by the Oxygen Consumption Rate (OCR) Glycolysis . Measured by the Extracellular Acidification Rate (ECAR)
MeasuringMitochondrialRespirationandGlycolysisinaMicroplateSensorProbesFiberOpticsXFCellCultureMicroplateAssayMediumSolidStateSensorCell MonolayerWell2Well 1
XF Cell Culture Microplate Assay Medium Solid State Sensor Cell Monolayer Sensor Probes Fiber Optics Well 1 Well 2 Measuring Mitochondrial Respiration and Glycolysis in a Microplate
MeasuringMitochondrialRespirationand Glycolysis in aMicroplateThe sensorAtransientmicro-chamberisformedThetransientmicro-chamberenablesrapidmeasurementofmitochondrialrespirationandglycolysisinminutesWell2Well 1
The transient micro-chamber enables rapid measurement of mitochondrial respiration and glycolysis in minutes Well 1 Well 2 Measuring Mitochondrial Respiration and Glycolysis in a Microplate The biocartridge is raised, bringing the system back to baseline The sensor the system back to baseline A transient microchamber is formed
MeasuringOxygenConsumptionRate(OCR)andExtracellularAcidificationRate (ECAR)1607.45每田思C27.40150?(0) 6H wwThecartridgeis raised1407.35吾C2-21C3OCR22bringingthecC2systemback1307.30to baselineCC2@nee?ECARC3Ca1207.25?C27.201002135674Time (rhin)TherateisCcalculatedfrom thepointsWell--1Well 2
Well 1 Well 2 Time (min) 100 120 130 140 150 160 1 2 3 4 7.20 7.25 7.30 7.35 7.40 7.45 pH 5 6 7 mm Hg (O 2 ) C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 C2 A temporary 2.28 µl microchamber is formed The biocartridge is raised, bringing the system back to baseline The biocartridge is raised, bringing the system back to baseline The cartridge is raised, bringing the system back to baseline ECAR OCR The rate is calculated from the points Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR)