-ti Ethanol Signal thanol Ethanol Ethanol 6 THMMR
THNMR YAN
典 3.30 H5 14·b H5 TOCSY M H2 NOESY H3 H3-H4 HI HMBC 570 H1-H2 5.70 W2(PPM) THMMR http://www.chem.queensu.ca/facilItieS/nmR/nmR/wEbcoUrsE/2Dnmrhtm
THNMR YAN H1 H1-H2 H2 H3 H3-H4 H4 http://www.chem.queensu.ca/FACILITIES/NMR/nmr/webcourse/2d_nmr.htm H51 H52 TOCSY NOESY HMBC
THR-VAL-LEU 0.5 B 1.5 TRY TO ASSIGN IT! I 5 min PPM THMMR T产 11
THNMR YAN THR-VAL-LEU TRY TO ASSIGN IT! 5 min
已… ……8…≤ a………已: Two ways of phase correcting a 2D COsY spectrum. diagonal peaks in-phase absorptive, crosspeaks antiphase dispersive(left or diagonal peaks in-phase dispersive, cross-peaks antiphase absorptive(right). The dispersive tails always tend to obscure near-by cross-peaks. THMMR
THNMR YAN Two ways of phase correcting a 2D COSY spectrum: diagonal peaks in-phase absorptive, crosspeaks antiphase dispersive (left); or diagonal peaks in-phase dispersive, cross-peaks antiphase absorptive (right). The dispersive tails always tend to obscure near-by cross-peaks
Disadvantage of COSY diagonal peaks are 90 degree out of phase compared to cross-peaks thus the diagonal peaks are usually phased to be dispersive so that the cross peaks would be absorptive Cross peaks only between directly coupled spins are observed Cross peaks show not only the active coupling but also passive coupling patterns. cosYis sensitive A normal Cosy can be obtained in 5 min for a modern NMR spectrometer! THMMR
THNMR YAN Disadvantage of COSY diagonal peaks are 90 degree out of phase compared to cross-peaks thus the diagonal peaks are usually phased to be dispersive so that the cross peaks would be absorptive. Cross peaks only between directly coupled spins are observed. Cross peaks show not only the active coupling but also passive coupling patterns. COSY is sensitive —— A normal COSY can be obtained in 5 min for a modern NMR spectrometer!