What is 2D NMR? 1)J分辨谱 J resolved spectroscopy) 将决定波谱共振位置的两个参数即化 学位移和J耦合分开 2)化学位移相关谱 (chemical shift correlation 同核相关谱 spectroscopy) 异核相关谱 将相互耦合的核、或具有化学交换的 核、或有相互弛豫的核相关联 3)多量子谱 (multiple quantum spectroscopy) 检测和应用多量子跃迁 THMMR
THNMR YAN What is 2D NMR? 1）J分辨谱 （J resolved spectroscopy） 将决定波谱共振位置的两个参数即化 学位移和J耦合分开. 2）化学位移相关谱 (chemical shift correlation spectroscopy) 将相互耦合的核、或具有化学交换的 核、或有相互弛豫的核相关联. 3）多量子谱 （multiple quantum spectroscopy） 检测和应用多量子跃迁. 同核相关谱 异核相关谱
62同核相关谱 HOMOZDJ COSY magnitude cosy, phase sensitive COsY DQF MQF)-COSY, RELAY, ECOSY, TOCSY NOESY THNMR AN
THNMR YAN 6.2 同核相关谱 HOMO2DJ COSY magnitude COSY, phase sensitive COSY, DQF(MQF)-COSY, RELAY, ECOSY, TOCSY NOESY
HOMO2DJ 180 t1/2 t/2 HOMO2DJ is used to separate chemical shifts and scalar coupling in different dimensions It involves an evolving spin-echo sequence, in which the intensity of multiplet lines are modulated by J coupling in t. THNMR YAN
THNMR YAN 90 180 t1 / 2 t1 / 2 HOMO2DJ • HOMO2DJ is used to separate chemical shifts and scalar coupling in different dimensions. • It involves an evolving spin-echo sequence, in which the intensity of multiplet lines are modulated by J coupling in t1
⊥: δ(f2)→ J (1) 0 HZ THMMR
THNMR YAN d (f2 ) J (f 1 ) 0 Hz
2D COSY(Correlated Spectroscopy) 90 2 H=C= O Cross peaks exist in the 2-D COSY C spectrum where there is spin-spin H coupling between orogens. THNMR YAN
2D COSY(Correlated Spectroscopy) N C H C H H C H O THNMR YAN t 1 90x t2 90x Cross peaks exist in the 2-D COSY spectrum where there is spin-spin coupling between hydrogens