/到 生物核磁共振波谱学 NMR in Biological Science THNMR AN
生物核磁共振波谱学 NMR in Biological Science THNMR YAN
Why learn NMr? A remarkable field One of the two leading technologies in structural biology Great contributions to biology A special window to the development of modern structural biology and biophysics THNMR AN
Why learn NMR? A remarkable field One of the two leading technologies in structural biology Great contributions to biology A special window to the development of modern structural biology and biophysics THNMR YAN
1.1A Remarkable field Nobel prize 1943年O.Stem物理学奖 测定质子磁矩 1944年.1.Rabi物理学奖 测定磁矩的共振方法 1952年FB0ch&E.M. Pruce物理学奖 发现核磁共振现象 1991年R.R.Emst化学奖 FTNMR 2D NMR THNMR AN
1.1 A Remarkable Field Nobel Prize 1943年 O. Stern 物理学奖 — 测定质子磁矩 1944年 I. I. Rabi 物理学奖 — 测定磁矩的共振方法 1952年 F.Bloch & E. M. Prucell 物理学奖 — 发现核磁共振现象 1991年 R. R. Ernst 化学奖 — FT NMR & 2D NMR THNMR YAN
1.2 One of the two leading technologies in structural biology Table 1 Annual publications of novel atomic resolution structures of proteins and nucleic acids(ref. 4) Method X-ray crystallography NMR Other methods Year (single crystals) (solution)(crystals, fibers) 1990 109 23 1991 123 36 1992 168 61 1993 207 59 1994 352 100 1995 394 102 1996 461 112 THNMR AN Ref: Wuthrich K Nature Structural Biology 5, 492-495 (1998
1.2 One of the two leading technologies in structural biology Ref: Wuthrich K. Nature Structural Biology 5, 492 - 495 (1998) THNMR YAN
1.2 One of the two leading technologies in structural biology PDB HOLDINGS (30-JUL-2001) MOLECULE TYPE EXPEREME PROTEINS, PEPTIDES. PROTEIN/NUCLEIC NUCLEIC CARBOHYD TOTAL AND VIRUSES ACID COMPLEXES ACIDS RATES NTAL TECHNIQU E X-RAY DⅠ FFRAC 11.630 580 14 12.780 TION AND OTHER NMR 1918 73 383 2.378 THEORET ICAL MODELIN TOTAL 13.836 648 986 18 15488 AN Ref: PDB ANNUAL REPORT, July 2000-June 2001
1.2 One of the two leading technologies in structural biology PDB HOLDINGS (30-JUL-2001) Ref: PDB ANNUAL REPORT, July 2000 - June 2001 MOLECULE TYPE EXPEREME NTAL TECHNIQU E PROTEINS, PEPTIDES, AND VIRUSES PROTEIN/NUCLEIC ACID COMPLEXES NUCLEIC ACIDS CARBOHYD RATES TOTAL X-RAY DIFFRAC TION AND OTHER 11,630 556 580 14 12,780 NMR 1,918 73 383 4 2,378 THEORET ICAL MODELIN G 288 19 23 0 330 TOTAL 13,836 648 986 18 15,488 THNMR YAN