HHS Publication No.(CDC)(99-xxxx) Biosafety in Microbiological and Biomedical Laboratories U.S.Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention and National Institutes of Health Fourth Edition April 1999
HHS Publication No. (CDC) (99-xxxx) Biosafety in Microbiological and Biomedical Laboratories U.S. Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention and National Institutes of Health Fourth Edition April 1999 U.S. GOVERNMENT PRINTING OFFICE WASHINGTON: 1999
Editors: ab2noMa d ph n Public He 1600 Cifton Road N.E Control and Prevention Atlanta,Georgia 30333 RobertW.McKinney,Ph.D Director,Division ofafety Health Room 1C0: Bethesda,Marylnd 20892
i Editors: Jonathan Y. Richmond, Ph.D. Director, Office o f Health a nd Safe ty Public Health Service Centers for Disease Control and Prevention 1600 Clifton Road N.E. Atlanta, Georgia 30333 Robert W. McKinney, Ph.D. Director, Division of S afety Public Health Service Nationa l Institutes of H ealth Building 31, Room 1C02 Bethesda, Maryland 20892
GUEST EDITORS Centers for Disease Control and Prevention RobertB.Craven,M.D. Arbovirus Disease Branch National Center for Infectious Disease Mark L.Eberhard,Ph.D. Chief National Center for Infectious Diseases Thomas Foks,Ph.D. Chief lV and Retr rology Branch Laboratory Research National Center for Infectious Diseases Bradford Kay.Dr.P.H. Senior Labo oratory Advisor rlogy and National Center for Infectious Diseases Richard C.Knudsen,Ph.D. Chief DW.J.Mahy.c. Director Disease of Viral and Rickettsial National Center for Infectious Diseases
ii GUEST EDITORS Centers for Disease Control and Prevention Robert B. Craven, M.D. Chief, Epidemiology Section Arbovirus Disease Branch National Center for Infectious Diseases Mark L. Eberhard, Ph.D. Chief Biology and Diagnostics Branch Division of Parasitic Diseases National Center for Infectious Diseases Thomas Folks, Ph.D. Chief HIV and Retrovirology Branch Division of AIDS, STD, and Laboratory Research National Center for Infectious Diseases Bradford Kay, Dr.P.H. Senior Laboratory Advisor Division of Bacteriology and Mycotic Diseases National Center for Infectious Diseases Richard C. Knudsen, Ph.D. Chief Laboratory Safety Branch Office of Hea lth and Safety Brian W . J. Mahy, Sc.D., Ph.D. Director Division of Viral and Rickettsial Diseases National Center for Infectious Diseases
C.J.Peters.M.D. Chief al Path National Center for Infectious Diseases Margaret A.Tipple,M.D. Chief National Institutes of Health John Bennett,MD. Chief.Mycobgy Section or Allergies and David Hackstadt,Ph.D. ain Laboratory Deborah E.Wilson,Dr.P.H. Chief,Occupational Health and Safety INDIVIDUAL GUEST EDITORS Jonathan Crane,A.LA. Architect Atlanta,GA PeterJ.Gerone,Sc.D. Director,Tulane Regional Primate Medical Center Covington,Louisiana .D.V.M Consultant Cary.NC
iii C.J. Peters, M.D. Chief Special Pathogens Branch National Center for Infectious Diseases Margaret A. Tipple, M.D. Chief External Activities Program Office o f Health a nd Safe ty National Institutes of He alth John Bennett, M.D. Chief, Mycology Section National Institute for Allergies and Infectious Diseases David Hackstadt, Ph.D. Microbiologist Rocky Mou ntain Laboratory Deborah E. Wilson, Dr.P.H. Chief, Occupational Health and Safety Branch Division of Safety INDIVIDUAL GUEST EDITORS Jonathan Crane, A.I.A. Architect Atlanta, GA Peter J. Gerone, Sc.D. Director, Tulane Region al Prima te Research Center Tulane University Medical Center Covington, Louisiana Thom as Ha mm , D.V.M ., Ph.D. Consultant Cary, NC
DebraL.Hunt,Dr.PH. Peter Jahring.Ph.D. Senior Research Scier ntist rMaryand Thomas Kost,Ph.D. icn Doparmont esearch TECHNICAL EDITOR Marie J.Murray Wl iv
iv Debra L. Hunt, Dr.P.H. Director, Biological Safety and Infection Control Duke University Medical Center Durham, North Carolina Peter Jahrling, Ph.D. Senior Research Scientist Disease Assessment Division USAM RIID Frederick, Maryland Thomas Kost, Ph.D. Section Head Molecular Sciences Department Glaxo Wellcome, Inc. Research Triangle Park, NC TECHNICAL EDITOR Marie J. Murray Writer-Editor Atlanta, GA