■ The proteome Protein complement of a genome vAriable In different cell and tissue types in same organism In different growth and developmental stages of organism I Dynamic Depends on response of genome to environmental factors Disease state m Drug challenge Growth conditions a Stress 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 6 The proteome The proteome – – Protein complement of a gen in complement of a genome Variable Variable – – In different cell and tissue types in same organism In different cell and tissue types in same organism – – In different growth and developmental stages of In different growth and developmental stages of organism organism Dynamic Dynamic – – Depends on response of genome to environmental Depends on response of genome to environmental factors factors Disease state Disease state Drug challenge Drug challenge Growth conditions Growth conditions Stress
人类了解自己的历史 整体水平→器官水平细胞水平□→分子水平 系统生物学 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 7
Content L. Introduction l. Proteomics and genomics ll. dentification and Quantification of proteins V Structural proteomics V. Identification of protein post- translational modifications VI. Protease degradomics Vll. Platforms for proteomics Vl. Mass Spectrometry IX. Post-MS Analysis X. Bioinformatics for proteomics 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 8 Content I. Introduction II. Proteomics and Genomics III. Identification and Quantification of Proteins IV. Structural Proteomics V. Identification of Protein Posttranslational Modifications VI. Protease degradomics: VII. Platforms for proteomics VIII. Mass Spectrometry IX. Post-MS Analysis X. Bioinformatics for Proteomics
Part Introduction 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 9 Part I. Introduction
二十世纪中期以来,随着DNA双 螺旋结构的提出和蛋白质空间结构的 X射线解析,开始了分子生物学时代, 对遗传信息载体DNA和生命功能的主 要体现者蛋白质的研究,成为生命科 学研究的主要内容。 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 10 二十世纪中期以来，随着DNA双 螺旋结构的提出和蛋白质空间结构的 X射线解析，开始了分子生物学时代， 对遗传信息载体DNA和生命功能的主 要体现者蛋白质的研究，成为生命科 学研究的主要内容。 二十世纪中期以来，随着DNA双 螺旋结构的提出和蛋白质空间结构的 X射线解析，开始了分子生物学时代， 对遗传信息载体DNA和生命功能的主 要体现者蛋白质的研究，成为生命科 学研究的主要内容