上帝充通大学 Shanghai Jiao Tong University PDA Medium(Potato Dextrose Agar) Autoclave 200 grams of sliced potato in 1000 mL of tap water.Filter through a hankerchief. To the turbid filtrate add 20 grams of glucose and 10 grams of agar.Add water to make 1 liter total volume. Autoclave PDA slants give good growth of most fungi, Agrobacterium,Nocardia,and many others. Shanghai Jiao Tong University
Shanghai Jiao Tong University PDA Medium (Potato Dextrose Agar) Autoclave 200 grams of sliced potato in 1000 mL of tap water. Filter through a hankerchief. To the turbid filtrate add 20 grams of glucose and 10 grams of agar. Add water to make 1 liter total volume. Autoclave PDA slants give good growth of most fungi, Agrobacterium, Nocardia, and many others
上帝充通大学 Shanghai Jiao Tong University SSW Medium (Synthetic Seawater Medium) MgS04.7H20...5.0g NaCl....... 30.0g MgC2.H20.....1.4g CaCl2.2H20.0.7g Yeast extract........0.5g Peptone....... ..0.5g Glycerol.............3 mL Water...........1000 mL This medium is excellent for growing most bacteria from the oceans. Shanghai Jiao Tong University
Shanghai Jiao Tong University SSW Medium (Synthetic Seawater Medium) MgSO4 .7 H2O.......... 5.0g NaCl........…....... 30.0g MgCl2.H2O............ 1.4g CaCl2 .2 H2O...... 0.7g Yeast extract........ 0.5g Peptone.....…....... 0.5g Glycerol............. 3 mL Water……..... 1000 mL This medium is excellent for growing most bacteria from the oceans
上泽充通大学 Shanghai Jiao Tong University Two most important nutritional factors Energy source能量来源 ·Chemical energy ·Light energy Carbon source碳源 ·Organic compounds ·Inorganic compounds ·CO,or carbonate Shanghai Jiao Tong University
Shanghai Jiao Tong University Two most important nutritional factors Energy source能量来源 • Chemical energy • Light energy Carbon source碳源 • Organic compounds • Inorganic compounds • CO2 or carbonate
上泽充通大学 Shanghai Jiao Tong University 4.3 Laboratory Culture of Microorganisms Pure Culture:a culture containing only a single kind of microorganism.纯培养 Solid culture media:culture media prepared in a semisolid form by the addition of a gelling agent to liquid media.固体培养基 Shanghai Jiao Tong University
Shanghai Jiao Tong University 4.3 Laboratory Culture of Microorganisms Pure Culture: a culture containing only a single kind of microorganism.纯培养 Solid culture media: culture media prepared in a semisolid form by the addition of a gelling agent to liquid media.固体培养基