上降充通大学 Shanghai Jiao Tong University @lron铁 Plays a major role in cellular respiration,being a key component of the cytochromes and iron-sulfur proteins involved in electron transport.在细胞的呼吸中有重 要作用，是电子传递链中细胞色素和铁硫蛋白 的重要组分 @Micronutrients((trace Elements)微量元素 They are metals,many of which play a structural role in various enzymes. Shanghai Jiao Tong University
Shanghai Jiao Tong University Iron铁 • Plays a major role in cellular respiration, being a key component of the cytochromes and iron-sulfur proteins involved in electron transport.在细胞的呼吸中有重 要作用, 是电子传递链中细胞色素和铁硫蛋白 的重要组分. Micronutrients (trace Elements)微量元素 • They are metals, many of which play a structural role in various enzymes
上泽充通大睾 Shanghai Jiao Tong University Growth Factors生长因子 Are organic compounds that are required in very small amounts and only by some cells. Include vitamins,amino acids,purines,and pyrimidines. Shanghai Jiao Tong University
Shanghai Jiao Tong University Growth Factors 生长因子 • Are organic compounds that are required in very small amounts and only by some cells. • Include vitamins, amino acids, purines, and pyrimidines
上帝充通大学 Shanghai Jiao Tong University Nutritional requirements of different microbes Different microorganisms can have vastly different nutritional requirements because they have different biosynthetic capacities E.coli:capable of synthesizing all organic compounds it needs Leuconostoc mesenteroides:need many externally provided organic compounds-extremely fastidious(nutritionally demanding)肠膜明串珠菌（需要极复杂营养的） Shanghai Jiao Tong University
Shanghai Jiao Tong University Nutritional requirements of different microbes Different microorganisms can have vastly different nutritional requirements because they have different biosynthetic capacities • E. coli: capable of synthesizing all organic compounds it needs • Leuconostoc mesenteroides: need many externally provided organic compounds-extremely fastidious (nutritionally demanding)肠膜明串珠菌（需要极复杂营养的）
上帝充通大学 Shanghai Jiao Tong University 4.2 Culture media:培养基 Culture media are the nutrient solutions used to grow microorganisms in the laboratory. Chemically defined the exact chemical composition is known化学限定的培养基 ·Synthetic media合成培养基 Minimal media限制性培养基 Basal media基础培养基 ©Chemically undefined非化学限定的培养基 ·Complex media复合培养基 ·Natural media天然培养基 Shanghai Jiao Tong University
Shanghai Jiao Tong University 4.2 Culture media培养基 Culture media are the nutrient solutions used to grow microorganisms in the laboratory. Chemically defined(the exact chemical composition is known)化学限定的培养基 • Synthetic media合成培养基 • Minimal media限制性培养基 • Basal media基础培养基 Chemically undefined非化学限定的培养基 • Complex media复合培养基 • Natural media天然培养基
帝克通大学 上 Shanghai Jiao Tong University Prepare 1L LB Medium (Luria-Bertani Medium): Per liter: Bacto-tryptone: 10g Bacto-yeast extract: 5g NaCl: 10g H,O: To 1 L Autoclave Shanghai Jiao Tong University
Shanghai Jiao Tong University Prepare 1L LB Medium (Luria-Bertani Medium): Per liter: Bacto-tryptone: 10g Bacto-yeast extract: 5g NaCl: 10g H2O: To 1 L Autoclave