TnphoA Transposon Created Tnphoa that randomly inserts phoa into target genes Hybrid proteins display alkaline phosphatase activity only if target gene encodes a periplasmic outer membrane or extracellular protein BIOL 533 Lecture 4
BIOL 533 12 Lecture 4 TnphoA Transposon • Created TnphoA that randomly inserts phoA into target genes • Hybrid proteins display alkaline phosphatase activity only if target gene encodes a periplasmic outer membrane or extracellular protein
TnphoA Transposon Use TnphoA Study topology of inner membrane proteins Bgal fusions expressed only in cytoplasm Identify genes that encode export proteins Exported proteins represent major class of virulence factors Number of additional delivery systems Remember analysis of groisman and heffron BIOL 533 Lecture 4
BIOL 533 13 Lecture 4 TnphoA Transposon • Use TnphoA: – Study topology of inner membrane proteins • βgal fusions expressed only in cytoplasm – Identify genes that encode export proteins • Exported proteins represent major class of virulence factors • Number of additional delivery systems • Remember analysis of Groisman and Heffron