Step5 Conversion of succinyl CoA to succinyl [catalyzed by succinyl CoA synthetase; the reaction requires inorganic phosphate and GDP(orADP)] CHa--co0 GDP+ P GTP CoA-SH cOO CHy CH C-S-COA succinyL CoA CH synthetase COo Succinyl-CoA S succinate △G°=-2.9kJ/mol
• Conversion of succinyl CoA to succinyl [catalyzed by succinyl CoA synthetase; the reaction requires inorganic phosphate and GDP(orADP)] Step5
Succinyl-CoA Succinyl-CoA CHs lis euccinyl-CoA Ribho-P-0-P-o-P-o- COA- SH GDP His Succinyl phosphate Figure 15-9 Intermediates in the succinyl-CoA synthetase reaction. In step (a phosphate replaces CoA in the enzyme-bound suceinyl-Ce forming a high-energy acyl phosphate. the succinyl phosphate donates its phosphate group a His residue on the enzyme, formin CH? nergy phosphohistidyl enzyme. In step 3 the phosphate germinal phosphate of GDP, formins up is transferred from the GTE
Succinyl-CoA
Step 6 Oxidation of succinyl to fumarate(catalyzed by succinyl dehydrogenase, the reaction involves FAD) CoO COO FAD FADH2 CHg CH CHg HC coo dlehystregnane coo Succinate Fumarate △G=0 k/nol
• Oxidation of succinyl to fumarate (catalyzed by succinyl dehydrogenase; the reaction involves FAD). Step 6
malonate O CH2 CHz CH Malonate Succinate
malonate
Hydration of fumarae to malate苹果酸 ( catalyzed by fumarase延胡索酸酶) Coo Coo CH Hgo HO-CH HC fumarase HC-H coo coo Fumarate te Malate △G°=-3.8kJ/mol
Hydration of fumarae to malate苹果酸 (catalyzed by fumarase 延胡索酸酶)