isomerization(异构化作用) of citrate to isocitrate( catalyzed by aconitase顺乌头酸酶) H2-COOH CH2-COOH HO-C-COOH H-C-COOH Ho-COOH HO-CH-COOH
Isomerization (异构化作用)of citrate to isocitrate (catalyzed by aconitase 顺乌头酸酶) • CH2 -COOH CH2 -COOH • HO-C-COOH H-C-COOH • CH2 -COOH HO-CH-COOH
socitrate formation 0+00900 H0C00 C-00c-00 aconitase c-00 C-00 H0c00 cis-Aconitate Isocitrate
Isocitrate formation
Oxidation of isocitrate to a-ketoglutarate (a-pA 戊二酸, catalyzed by isocitrate dehydrogenase; the reaction requires NAd+) COOH NAD+ NADH+H+ COOH CH CH H-C-COOH CH 2 HO-C-H co, CO COOH COOH
Oxidation of isocitrate to α –ketoglutarate（α-酮 戊二酸, catalyzed by isocitrate dehydrogenase; the reaction requires NAD+ ） • COOH COOH • CH2 CH2 • H-C-COOH CH2 • HO-C-H CO • COOH COOH NAD+ NADH+H+ CO2
a-ketoglutarate ClHr-c00. NADP),NADPH+H isocitrate 00 0-c-00 0c-00
α –ketoglutarate
Step 4 Oxidation of a -ketoglutarate to succinyl CoA (catalyzed by the a -ketoglutarate dehydrogenase complex the reaction requires a NAD+) CHi-CO0 CoA-SH NAD NADH CHo-cOO CH +cO ketoglutarate C-S-COA dehydrogenase 0 kEtoglutarate Succinyl-COA
• Oxidation of α –ketoglutarate to succinyl CoA (catalyzed by the α –ketoglutarate dehydrogenase complex ; the reaction requires a NAD+ ) Step 4