General structure: two sta cked, inv erted cylinders 8.4 Hsp60/GroEL forms an oligomeric ring structure der consist Top view: 7-fold symmetry with central hole 4.5 Figure 8.7-2 GroEL forms an oligomer of two rings, each Side view: rings have inverted in orientations comprising a hollow TOp ring 45 cylinder made of 7 subunits odom nng 消当
Figure 8.7-2 GroEL forms an oligomer of two rings, each comprising a hollow cylinder made of 7 subunits. 8.4 Hsp60/GroEL forms an oligomeric ring structure
8.4 Hsp60/GroEL GOES folded forms an oligomeric substrate 3 nm ring structure 75nm Figure 8. Two rings of GroeL associate back to Prox m al GroEL back to form a hollow cylinder. GroeS forms a dome that covers the Distal groel central cavity on one side Protein substrates bind to the cavity in the distal ring 消当
Figure 8.8 Two rings of GroEL associate back to back to form a hollow cylinder. GroES forms a dome that covers the central cavity on one side. Protein substrates bind to the cavity in the distal ring. 8.4 Hsp60/GroEL forms an oligomeric ring structure
8.4 Hsp60/GroEL forms an oligomeric ring structure ATP 部 Figure 8.9 Protein folding occurs in the Proximal ATP hydrolysis occurs proximal groel ring and requires atp ATP Release of substrate Distal ATP hydrolysis drives release of GroES and substrate and groes requires atP hydrolysis in the distal ring 消当
Figure 8.9 Protein folding occurs in the proximal GroEL ring and requires ATP. Release of substrate and GroES requires ATP hydrolysis in the distal ring. 8.4 Hsp60/GroEL forms an oligomeric ring structure
ORGANELLE 8.5 Post-translational Proteln: passes througl iermhiran and: leader membrane insertion sequece is: cleaved depends on leader Mature: protein sequences Figure 8.10 Leader sequences allow proteins to recognize eader sequence binds mitochondrial or to receptor: on organelle chloroplast surfaces membrane by a post-translational CYTOSOL process 消当
Figure 8.10 Leader sequences allow proteins to recognize mitochondrial or chloroplast surfaces by a post-translational process. 8.5 Post-translational membrane insertion depends on leader sequences
8.5 Post-translational membrane insertion depends on leader sequences Initiation Cleavage Hydrophobic Polar 日asic 网四國國回國國回四巴四画回國回四回∞匚 Figure 8. 12 The leader sequence of yeast cytochrome c oxidase subunit iv consists of 25 neutral and basic amino acids. The first 12 amino acids are sufficient to transport any attached polypeptide into the mitochondrial matrix 消当
Figure 8.12 The leader sequence of yeast cytochrome c oxidase subunit IV consists of 25 neutral and basic amino acids. The first 12 amino acids are sufficient to transport any attached polypeptide into the mitochondrial matrix. 8.5 Post-translational membrane insertion depends on leader sequences