2.3 How DNA has 3 target sites Mutation eliminates n region 1 target site variable are individual v→AA genomes? ↓s Cleavage generates leav age generate s 2 internal fragments nternal fragment ↓ fragment Vw Figure 6 fragment A fragment C A point mutation Electrophoresis that affects a restriction site Is C detected by a dufference in restriction A. Fragment =A+B combined fragments 消当
Figure 2.6 A point mutation that affects a restriction site is detected by a difference in restriction fragments. 2.3 How variable are individual genomes?
2. 3 How Parent are heterozygous variable is homozygous for C are individual E2 inherit. from one parent B or C from other parent D genomes? Allele A Figure 2.7 Restriction site polymorphisms are Allele B Inherited according Allele C to Mendelian rules Allele D 二 Four alleles for a restriction marker are found in all possible pairwise combinations, and segregate Independently at each 消当 generation. Photograph kindly provided by Ray White
Four alleles for a restriction marker are found in all possible pairwise combinations, and segregate independently at each generation. Photograph kindly provided by Ray White. 2.3 How variable are individual genomes? Figure 2.7 Restriction site polymorphisms are inherited according to Mendelian rules
Genetic cross 2. 3 How variable are individual genomes? 35% 35 Parental types Figure 8 A restriction polymorphism can be used as a genetic marker to measure recombination distance from a phenotypic marker(such 15% 15 as eve color). The figure simplifies the situation by Recombinants showing only the dna bands corresponding to the allele of the other genome in a diploid 消当 Restriction marker is 30 map units from eye color marker
Figure 2.8 A restriction polymorphism can be used as a genetic marker to measure recombination distance from a phenotypic marker (such as eye color). The figure simplifies the situation by showing only the DNA bands corresponding to the allele of the other genome in a diploid. 2.3 How variable are individual genomes?
2. 3 How variable are individual screen DNA pattems of Screen dNA patterns of atients with dise nomal people as control genomes Figure 29 囗囗囗 If a restriction Band is same in patient and nomal marker is associated Unlinked polymorphism varies with a phenotypIc in all samples haracteristic the Band is common to patients restriction site must be located near the Band is aom mon to nomal p)國 gene responsible for the phenotype The mutation changing the band that Is common in normal people into the 消当 band that is common in patients Is very closely linked to the dr
Figure 2.9 If a restriction marker is associated with a phenotypic characteristic, the restriction site must be located near the gene responsible for the phenotype. 2.3 How variable are individual genomes? The mutation changing the band that is common in normal people into the band that is common in patients is very closely linked to the disease gene
2. 3 How variable are individual screen DNA pattems of Screen dNA patterns of atients with dise nomal people as control genomes Figure 29 囗囗囗 If a restriction Band is same in patient and nomal marker is associated Unlinked polymorphism varies with a phenotypIc in all samples haracteristic the Band is common to patients restriction site must be located near the Band is aom mon to nomal p)國 gene responsible for the phenotype The mutation changing the band that Is common in normal people into the 消当 band that is common in patients Is very closely linked to the dr
Figure 2.9 If a restriction marker is associated with a phenotypic characteristic, the restriction site must be located near the gene responsible for the phenotype. 2.3 How variable are individual genomes? The mutation changing the band that is common in normal people into the band that is common in patients is very closely linked to the disease gene