2.2 Genes Individual ndividual Total DNA A-fragments B-fragments digeste 已 can be are digested are digested w ith both with enzymes with enzyme a enzymes mapped by吕吕 吕吕吕 restriction xx cleavage ↓ Flgure 22 Double digests define the cleavage positions 000 of one enzyme with 哥卧国 600 regard to the other 50 消当
Figure 2.2 Double digests define the cleavage positions of one enzyme with regard to the other. 2.2 Genes can be mapped by restriction cleavage
A-2100—) The A-2100 2.2 Genes can fragm ent identifies 2 ing sites& 1 B-cutt ing site be mapped by 1900 restriction 21 A-digest fragments cleavage Double 2:P with the fragment Figure 2 3 1900 B B-digest fragments/ to the right map A restriction map can be A A constructed by Overlap with日 1200 extends m elating the A 分 o left; overlap 6“少 A-1400extends map to right fragments and B 72U 2500 fragments through the overlaps seen 700A Left end of map is with double digest identi fied by A- fragments ←-1005←m1个 fragm ents right end is identi fed by z0…>B-2500 →)Be-10→ A-500and日-1300 消当
Figure 2.3 A restriction map can be constructed by relating the Afragments and Bfragments through the overlaps seen with double digest fragments. 2.2 Genes can be mapped by restriction cleavage
2.2 Genes can be mapped by restriction cleavage 圆用< tion ma restriction fragments are identified by fragments identified on gel their possession of AG〔 broken labeled end. each HA-B-C-D-E E-F fragment directly shows the distance 目CBE of a cutting site A-B-C from the end A-日 Successive 凸 Fragments Increase In length by the distance between diacent restriction Labeled end sites 消当
Figure 2.4 When restriction fragments are identified by their possession of a labeled end, each fragment directly shows the distance of a cutting site from the end. Successive fragments increase in length by the distance between adjacent restriction sites. 2.2 Genes can be mapped by restriction cleavage
2.2 Genes can be mapped by restriction cleavage 10020190060805( Figure 2.5 A restriction map is a linear sequence of sites separated by defined distances on DNA. The map identifies the sites cleaved by enzymes A and B, as 请菲大 ned by the individual fragments produced by the single and double digests
Figure 2.5 A restriction map is a linear sequence of sites separated by defined distances on DNA. The map identifies the sites cleaved by enzymes A and B, as defined by the individual fragments produced by the single and double digests. 2.2 Genes can be mapped by restriction cleavage
2.3 How variable are individual genomes? Haplotype is the particular combination of alleles in a defined region of some chromosome. in effect the genotype in miniature. Originally used to described combinations of MHC alleles. it now may be used to describe particular combinations of RFLPS SNP (sIngle nucleotide polymorphism) Is any site at which a single nucleotide has changed when two (haploid) genomes are compared 消当
Haplotype is the particular combination of alleles in a defined region of some chromosome, in effect the genotype in miniature. Originally used to described combinations of MHC alleles, it now may be used to describe particular combinations of RFLPs. SNP (single nucleotide polymorphism) is any site at which a single nucleotide has changed when two (haploid) genomes are compared. 2.3 How variable are individual genomes?